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Plasmid#234953PurposeHuman codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmidDepositorInserthuman codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo
Plasmid#234954PurposeHuman codon optimized T4 DNA Ligase with N-terminal F0ATP MLS expressing plasmidDepositorInserthuman codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of subunit 9 of F0-ATP synthase
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP
Plasmid#233442PurposePiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)DepositorInsertFKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato
Plasmid#234951PurposeHuman codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmidDepositorInsertHuman codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterEF1AAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP1199 - pAAV-AiE2010m-minBG-IRES2-iCre-HGHpA
Plasmid#230585PurposeAiE2010m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertiCre
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP982 - pAAV-AiE2004m-minBG-IRES2-iCre-HGHpA
Plasmid#230568PurposeAiE2004m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertiCre
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mHFE(H67D)-IRES-GFP-WPRE-pA
Plasmid#233040PurposeTo express mouse homeostatic iron regulator (HFE)(H67D) from an EF1a promoter. It also encodes GFP after an IRES sequenceDepositorInsertmouse homeostatic iron regulator with H67D mutation (Hfe Mouse)
UseAAVTagsGFPExpressionMutationH67DPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Const.5 EGFP-P2A-dTomato-IRES-Luc-PS9
Plasmid#231909PurposeFor packaging EGFP, dTomato and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)DepositorInsertEGFP-P2A-dTomato-IRES-Luc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP1666 - pAAV-AiE0486m-minBG-IRES2-iCre-HGHpA
Plasmid#220710PurposeAiE0486m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cellsInsertiCre
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry
Plasmid#232246PurposeMammalian expression of delta73-82 REX1-GFP-IRES-mCherryDepositorInsertdelta73-82 REX1-GFP-IRES-mCherry (Zfp42 Mouse)
UseTagsAcGFP1ExpressionMammalianMutationdelta73-82PromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro human-GFP-RLIM-IRES-mCherry
Plasmid#232243PurposeMammalian expression of RLIM-GFP-IRES-mCherryDepositorInsertRLIM-GFP-IRES-mCherry (RLIM Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro GFP-REX1[degron]-IRES-mCherry
Plasmid#232240PurposeMammalian expression of REX1[degron]-GFP-IRES-mCherryDepositorInsertREX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)
UseTagsAcGFP1ExpressionMammalianMutationREX1 (aa 70-85)PromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)
Plasmid#231233PurposeMYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationTAD deleted (delta1-143); numbering based on sequ…PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-FLEX-macaque OPN4-IRES-EGFP
Plasmid#223253PurposeAAV construct for Cre-dependent (FLEX) expression of macaque melanopsin (Opn4, short isoform, GenBank:OP856641; hSyn promoter) and of EGFP via an IRES.DepositorInsertmacaque OPN4-IRES-EGFP (OPN4 )
UseAAVTagsExpressionMutationPromoterhuman Synapsin 1 (hSyn)Available sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry
Plasmid#226104PurposeStability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cellsDepositorInsertCOX8A (COX8A Human)
UseTagsGFPExpressionMammalianMutationencodes only for mitochondrial targeting sequence…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-cDELE1(142-515)-GFP-IRES-mCherry
Plasmid#226102PurposecDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cellsDepositorInsertDELE1 (DELE1 Human)
UseTagsGFPExpressionMammalianMutationonly encodes for residues 142-515 of DELE1 corres…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP
Plasmid#225490PurposeNeuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseNeuronal expressionTagsExpressionMammalianMutationPromoterhSynAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)
Plasmid#224532PurposeA Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyADepositorInsertIRES-H2A-mCherry-SV40pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221076PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertGGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221077PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertCCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only