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We narrowed to 24,475 results for: crispr

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Showing: 2101 - 2120 of 24475 results

  1. pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Plasmid
    #101203
    Purpose
    Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…
    Promoter
    T7
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    101203-plasmid-map-sequence-id-197572

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  3. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available Since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

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  6. pDD285

    Plasmid
    #66826
    Purpose
    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    mKate2-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized mKate2
    Expression
    Worm
    Available Since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pAAV-i53

    Plasmid
    #92170
    Purpose
    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    i53
    Use
    AAV
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    CMV
    Available Since
    July 13, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    92170-plasmid-map-sequence-id-182617

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  9. eSpCas9(1.1)

    Plasmid
    #138555
    Purpose
    Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    eSpCas9(1.1)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138555-plasmid-map-sequence-id-274761

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  16. pCX0393

    Plasmid
    #205984
    Purpose
    Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Inserts
    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    truncated CRISPR array
    Tags
    14xHis and TwinStrep-tag
    Expression
    Bacterial
    Promoter
    T7 (proteins), pJ23119 (crRNA)
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205984-plasmid-map-sequence-id-416238

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Showing: 2101 - 2120 of 24475 results