165,971 results

1. pU6-GG-gRNA

   Plasmid

   #226860

   Purpose

   Human gRNA expression vector backbone with BsmBI Golden Gate site

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   Sp-gRNA
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-LUC2CP/intron/ARE

   Plasmid

   #62858

   Purpose

   splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

   Depositor

   Gideon Dreyfuss

   Article

   Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

   Insert

   Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   insertion of chimeric intron at nueotide poclsiti…

   Promoter

   CMV

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. FIRES-PBCS-CLV3

   Plasmid

   #228697

   Purpose

   Sensor cassette FIRE1, FIRE fluorescent ratiometric sensor

   Depositor

   Carolyn Sangokoya

   Article

   Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

   Insert

   FIRE1 SENSOR
   

   Use

   Synthetic Biology and Unspecified

   Tags

   mClover3

   Expression

   Mammalian

   Promoter

   hpgk

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. FIREC-PBCS-MC2

   Plasmid

   #228698

   Purpose

   Control cassette FIRE1, FIRE fluorescent ratiometric sensor

   Depositor

   Carolyn Sangokoya

   Article

   Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

   Insert

   FIRE1 CONTROL
   

   Use

   Synthetic Biology and Unspecified

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Parkin-IRES-zeo

   Plasmid

   #61727

   Purpose

   Expression of human Parkin fused to mCherry

   Depositor

   Douglas Green, Stephen Tait

   Article

   Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

   Insert

   Parkin (PRKN Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Paxillin-22

   Plasmid

   #55114

   Purpose

   Localization: Focal Adhesions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Paszek et al Nat Methods. 2012 Jul 1;9(8):825-7. doi:

   Insert

   Paxillin
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. mPA-GFP-CD81-10

   Plasmid

   #57124

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   CD81 (CD81 Human)
   

   Tags

   mPA-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. YFP NLS Beta-Actin G13R

   Plasmid

   #60615

   Purpose

   Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

   Depositor

   Primal de Lanerolle

   Article

   Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

   Insert

   Beta-actin (ACTB Human)
   

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   G13R

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. GRM1-DuET

   Plasmid

   #213300

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GRM1 (GRM1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. plenti-CAG-gate-FLAG-IRES-GFP

    Plasmid

    #107398

    Purpose

    Lentiviral gateway destination vector

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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