165,971 results

1. pDEST-CMV 3xFLAG-LC3B-GFP

   Plasmid

   #123107

   Purpose

   Expresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAJ075_hsCRBN

   Plasmid

   #124214

   Purpose

   Insect cell expression vector for His6 tagged hsCRBN

   Depositor

   Eric Fischer

   Article

   Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

   Insert

   Cereblon (CRBN Human)
   

   Tags

   His6-TEV

   Expression

   Insect

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO ChETA-EYFP

   Plasmid

   #26968

   Purpose

   AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   hChR2(E123T/H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T, H134R

   Promoter

   EF1alpha

   Available Since

   Jan. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_TNFRSF17_WT

   Plasmid

   #82263

   Purpose

   Gateway Donor vector containing TNFRSF17 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TNFRSF17 (TNFRSF17 Human)
   

   Use

   Gateway entry vector

   Mutation

   N81S; K86E

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. HER2 CA (V659E)

   Plasmid

   #16259

   Depositor

   Mien-Chie Hung

   Article

   Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

   Insert

   HER2 (ERBB2 Human)
   

   Expression

   Mammalian

   Mutation

   Constitutively active: V659E

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-V5-BRSK1_IDG-K

   Plasmid

   #135270

   Purpose

   Gateway destination clone of BRSK1 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-BRSK1 (BRSK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-L1PA1CH_blast rescue

   Plasmid

   #69611

   Purpose

   Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts

   Depositor

   Astrid Roy-Engel

   Insert

   L1PA1
   

   Tags

   blast rescue cassette

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPP381

   Plasmid

   #158798

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   dCasPhi-2
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   RuvC-inactivating mutation D394A

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MEK1C222

   Plasmid

   #164638

   Purpose

   Bacterial expression plasmid for His6-MEK1C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   G(-19)F/S222C/C277S/C376S

   Promoter

   T7

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p201N Cas9

    Plasmid

    #59175

    Purpose

    Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    nptII
    

    Use

    CRISPR

    Promoter

    2x35S and StUbi-3P

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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