165,971 results

1. pEMS1982

   Plasmid

   #49113

   Purpose

   AAV plasmid with CLDN5 (Ple261) promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

   Insert

   ssAAV-Ple261-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CLDN5

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pJRH029

   Plasmid

   #171060

   Purpose

   Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   Gag-spyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x SV40 NLS and 3x FLAG tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. peGFP C2-IST1

   Plasmid

   #218582

   Purpose

   Expresses GFP-tagged human IST1 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   IST1 (IST1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCX-DI-2A

   Plasmid

   #74671

   Purpose

   Mammalian expression of DI-2A (hCD73/F2A/hCD39)

   Depositor

   Roberto Giovannoni

   Article

   De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

   Inserts

   CD73 (NT5E Human)
   CD39 (ENTPD1 Human)
   

   Tags

   F2A

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKI1.1R

   Plasmid

   #85808

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Plant

   Promoter

   AtRPS5A

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

   Plasmid

   #111568

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2w/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Has Service

   AAV5

   Insert

   Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
   

   Use

   AAV

   Tags

   mCherry

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-EF1a-BbChT

   Plasmid

   #45186

   Depositor

   Dawen Cai, Joshua Sanes

   Article

   Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

   Has Service

   AAV9

   Insert

   pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE
   

   Use

   AAV; Adeno-associated viral

   Expression

   Mammalian

   Promoter

   human EF1a

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCF525-EF1a-Hygro-P2A-mCherry-lenti

   Plasmid

   #115796

   Purpose

   Expresses mCherry in human cells, contained in a lentiviral vector.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Promoter

   EF-1a promoter

   Available Since

   Oct. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENN.AAV.CMVs.Pl.Cre.rBG

   Plasmid

   #105537

   Purpose

   AAV expression of Cre from CMV promoter

   Depositor

   James M. Wilson

   Has Service

   AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_EF1a_TauFRET_3xKQ_wpre

    Plasmid

    #207522

    Purpose

    Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

    Depositor

    Bradley Hyman

    Article

    Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

    Insert

    TauFRET_P301L_3xKQ
    

    Use

    Lentiviral

    Tags

    NeonGreen and mTurquoise2

    Mutation

    P301L; K311Q; K317Q; K321Q

    Promoter

    EF1a

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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