We narrowed to 13,297 results for: EGFP

1. EGFP-SopB(68-554)

   Plasmid

   #183663

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 68-554

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. His6-EGFP-cPHx1

   Plasmid

   #183674

   Purpose

   PtdIns(3,4)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLEKHA1 (PLEKHA1 Human)
   

   Use

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 169-329

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SopB(C460S)-EGFP

   Plasmid

   #183656

   Purpose

   C-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C460S

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-SopB(C460S)

   Plasmid

   #183658

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C460S

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. BiDi-[mp-mEGFP]mp-mCherry2

   Plasmid

   #182870

   Purpose

   Bi-directional expression vector of monomers with plasma membrane localization signal

   Depositor

   Salvatore Chiantia

   Article

   Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

   Insert

   myr-palm-mEGFP, myr-palm-mCherry2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. myr-palm-mEGFP(4x)

   Plasmid

   #182873

   Purpose

   Homo-tetramer expression vector with plasma membrane localization signal

   Depositor

   Salvatore Chiantia

   Article

   Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

   Insert

   myr-palm-mEGFP(4x)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p{3P3-eGFP::GW,attB}

   Plasmid

   #182167

   Purpose

   fluorescent reporter gene with intron containing Gateway cloning cassette

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EGFP.T2A.NCLX.3'UTR

   Plasmid

   #181872

   Purpose

   Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Inserts

   solute carrier family 8 member B1 (Slc8b1 Mouse)
   EGFP
   3'UTR of solute carrier family 8 member B1
   

   Use

   AAV and Adenoviral

   Tags

   HA, T2A, and myc

   Expression

   Mammalian

   Mutation

   includes a large portion of the Slc8b1 3'UTR…

   Promoter

   synthetic hybrid CAG promoter

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFX-Lifeact-EGFP

   Plasmid

   #174477

   Purpose

   Express EGFP with actin-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with actin-targeting sequence
   

   Use

   Tags

   Lifeact (N-terminal 17 amino acids from S. Cerevi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-POLBHR-eGFP

    Plasmid

    #176064

    Purpose

    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    EGFP-PolB (POLB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    pUC with endogenous SapI mutated out, PAM mutatio…

    Promoter

    N/A

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CTAG_eGFP-GHtag-HDEL

    Plasmid

    #136096

    Purpose

    FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. SITS-Cavin4b-EGFP

    Plasmid

    #176019

    Purpose

    Leishmania cell free expression of zebrafish Cavin4b-EGFP. Parton lab clone GRJ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Cavin4b (cavin4b Zebrafish)
    

    Use

    Leishmania cell free expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SITS-EGFP-Bin1a

    Plasmid

    #176020

    Purpose

    Leishmania cell free expression of zebrafish EGFP-Bin1a. Parton lab clone GRQ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1a
    

    Use

    Leishmania cell free expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. SITS-EGFP-Bin1b

    Plasmid

    #176021

    Purpose

    Leishmania cell free expression of zebrafish EGFP-Bin1b. Parton lab clone GRS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Leishmania cell free expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SITS-Cavin4a-EGFP

    Plasmid

    #176018

    Purpose

    Leishmania cell free expression of zebrafish Cavin4a-EGFP. Parton lab clone GRK

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Cavin4a (cavin4a Zebrafish)
    

    Use

    Leishmania cell free expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FKBP-EGFP-RanGAP*

    Plasmid

    #175238

    Purpose

    Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FKBP-EGFP-RanGAP

    Plasmid

    #175236

    Purpose

    Bacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIVT-EGFP-ratCENP-C

    Plasmid

    #174445

    Purpose

    in vitro transcription template for rat CENP-C (N-ter EGFP tagged)

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Centromere protein C
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    T7 SP6

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIVT-EGFP-pumilioCENP-C

    Plasmid

    #174446

    Purpose

    in vitro transcription template for R. pumilio CENP-C (N-ter EGFP tagged)

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Centromere protein C
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    T7 SP6

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pnCS_mEGFP-DSep2_Peanut-Strep

    Plasmid

    #174496

    Purpose

    bacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila Peanut

    Depositor

    Manos Mavrakis

    Article

    Mavrakis et al Methods Cell Biol. 2016;136:199-220. doi

    Inserts

    Sep2 (Sep2 Fly)
    peanut (pnut Fly)
    

    Use

    Tags

    Strep and mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRabies-EGFP-rtTAn

    Plasmid

    #172119

    Purpose

    Expression of EGFP and rtTA-InteinN in Rabies virus infected cells

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    EGFP, rtTAn-InteinN
    

    Use

    Rabies virus

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TetO::EGFP-C-Meikin

    Plasmid

    #174706

    Purpose

    Doxycycline inducible expression of human C-Meikin Separase cleavage fragment

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    155-373

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGFP-Flag-stm2585

    Plasmid

    #174397

    Purpose

    Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags.

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA
    

    Use

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SITS-ccdb-EGFP

    Plasmid

    #118752

    Purpose

    Multisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEX

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ccdb
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SITS-EGFP-ccdb

    Plasmid

    #118628

    Purpose

    Multisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZ

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ccdb
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-Flag-stm2585_L139*

    Plasmid

    #174400

    Purpose

    S. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA
    

    Use

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Mutation

    L139* (Truncation after 139th amino acid in sarA)

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-Flag-stm2585_S130A

    Plasmid

    #174399

    Purpose

    Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAP

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA
    

    Use

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Mutation

    S130A

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-Flag-stm2585_Y167F

    Plasmid

    #174398

    Purpose

    Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQ

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA
    

    Use

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Mutation

    Y167F

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAFNF-LifeAct-EGFP

    Plasmid

    #163608

    Purpose

    Expresses LifeAct fused to EGFP Flp-dependently in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    LifeAct
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EGFP-uTEV1-SQSfl

    Plasmid

    #171010

    Purpose

    HiLITR protease with full-length SQS targeting information (ER)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-SQS(FL)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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