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pACT2-CAS9c
Plasmid#51055PurposeExpress Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.DepositorInsertCas9c
UseCRISPRTagsSV40 NLSExpressionBacterial and YeastMutationPromoteryeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pTET2-RlucCP
Plasmid#198352PurposePart of Firefly reporter system, encodes Renilla luciferaseDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterpTET2Available sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3
Plasmid#173936PurposeBacterial expression of PARP1 lacking Zn3 domainDepositorInsertPARP1delZn3 (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 214-373; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2
Plasmid#178575PurposeLentiviral vector for GFAP::Opto-vTrapDepositorInsertCRY2PHR-P2A-CIBN-mCherry-VAMP2
UseLentiviralTagsExpressionMutationPromoterGFAPAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-NDT1
Plasmid#133254PurposeExpress FLAG-tagged NDT1 in mammalian cellsDepositorInsertNDT1 (YIA6 Budding Yeast)
UseRetroviralTags3xFLAGExpressionMammalianMutationcodon-optimized for expression in human cellsPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-MCART6
Plasmid#133251PurposeExpress FLAG-tagged MCART6 in mammalian cellsDepositorInsertMCART6 (SLC25A53 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationcodon-optimized for expression in human cellsPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Med29 pBacPAK8
Plasmid#15369DepositorInsertMed29 (MED29 Human)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MEKK4alpha WT
Plasmid#12187DepositorInsertMEKK4 (Map3k4 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pASPIre1
Plasmid#154842PurposeDerivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sitesDepositorInsertBxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
UseTagsExpressionBacterialMutationPromoterPrhaAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDest-636
Plasmid#159574PurposeGateway Destination vector for protein expression in baculovirusDepositorTypeEmpty backboneUseTagsHis6-MBPExpressionInsectMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-MCART2
Plasmid#133250PurposeExpress FLAG-tagged MCART2 in mammalian cellsDepositorInsertMCART2 (SLC25A52 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationcodon-optimized for expression in human cellsPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
plc242-GFP-WDR24
Plasmid#87050Purposemammalian expression of WDR24DepositorInsertWDR24 (WDR24 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pASPIre3
Plasmid#154844PurposeDerivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this studyDepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-Cre-2A-mCherry-FKF
Plasmid#119868PurposeDonor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertNLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
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LightR-iCre-miRFP670
Plasmid#162158PurposeExpresses LightR-iCre-miRFP670 in mammalian cellsDepositorInsertLightR-iCre-miRFP670
UseTagsNLS and miRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only
