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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,042 results for: GRI

Showing: 2121 - 2140 of 3042 results

  1. p-mCherry-C1-iCoChR++

    Plasmid
    #98173
    Purpose
    artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iCoChR++ gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    A59S, E83N, E90Q, E101S, Q117R, E123S, V242R, T24…
    Promoter
    CMV (+enhancer)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98173-plasmid-map-sequence-id-182757

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  2. pETSUMO2_Bradyrhizobium-bGSDM

    Plasmid
    #212126
    Purpose
    For expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Science. 2022 Jan 14;375(6577):221-225.
    Insert
    Bradyrhizobium bGSDM
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    Jan. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    212126-plasmid-map-sequence-id-418775

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  6. VanGlow-GL-DSCP

    Plasmid
    #83338
    Purpose
    for Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Article
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    GFP::Luciferase(nls)
    Expression
    Insect
    Mutation
    Promoter
    Drosophila Synthetic Core Promoter
    Available since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83338-plasmid-map-sequence-id-158645

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  7. AA290

    Plasmid
    #215949
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD55_v4; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215949-plasmid-map-sequence-id-431450

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  8. AA286

    Plasmid
    #215948
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD47_v6; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215948-plasmid-map-sequence-id-431417

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  16. B4GALT1-Dox

    Plasmid
    #124633
    Purpose
    Doxycycline-inducible B4GALT1 expression in mammalian cells
    Depositor
    Ron Weiss
    Article
    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038
    Inserts
    Beta-(1,4)-Galactosyltransferase 1
    Tetracycline repressor A3 mutant
    TagFP635
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight and hEF1a
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124633-plasmid-map-sequence-id-248076

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  20. p-mCherry-C1-iC++

    Plasmid
    #98165
    Purpose
    artificial anion conducting channelrhodopsin (aACR). Activation max 488 nm; off-kinetics 35 ms. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iC++ gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, V242R, T24…
    Promoter
    CMV (+enhancer)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98165-plasmid-map-sequence-id-182714

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Showing: 2121 - 2140 of 3042 results