We narrowed to 8,589 results for: Set

1. pDT305

   Plasmid

   #128801

   Purpose

   Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone

   Depositor

   Bradley Bernstein

   Article

   Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

   Insert

   dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
   

   Use

   CRISPR and Lentiviral

   Promoter

   hUBC promoter

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBWB162

   Plasmid

   #140634

   Purpose

   pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

   Depositor

   Keith Tyo

   Article

   Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

   Insert

   mCherry
   

   Expression

   Bacterial

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWALIUM-intron 3x UAS attB

   Plasmid

   #206360

   Purpose

   includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

   Depositor

   Erdem Bangi

   Article

   Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCBL101gw

   Plasmid

   #199725

   Purpose

   A pVS1-based T-DNA binary vector and destination vector for Gateway cloning with NPTII as a plant selectable marker gene.

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   attR1/R2 cassette
   

   Expression

   Plant

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-SC35-cMyc

   Plasmid

   #44721

   Depositor

   Kathleen Scotto

   Article

   Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

   Insert

   SC35 (SRSF2 Human)
   

   Tags

   his and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPL5071_TEF1*-Cre_URA3

   Plasmid

   #60930

   Purpose

   Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Promoter

   mutant TEF1

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKM446 (Flag-DAS+4))

   Plasmid

   #108321

   Purpose

   Orbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   attB-Flag-DAS+4
   

   Use

   Orbit integrating plasmid

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSelAct-Express

   Plasmid

   #174375

   Purpose

   Plasmid for integrative expression, pUC19 backbone, Apr resistant casette, pCMP1::eGFP expression cassette, gateway compatible

   Depositor

   Gitta Coaker

   Article

   Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

   Type

   Empty backbone

   Use

   Expression vector for targeted integration

   Promoter

   pCMP1

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Prab-3::NLS::GFP

   Plasmid

   #68120

   Purpose

   Nuclear Localized GFP for pan-neuronal expression in C. Elegans

   Depositor

   Andrew Leifer

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

   Insert

   GFP
   

   Expression

   Worm

   Promoter

   rab-3

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM444

    Plasmid

    #108319

    Purpose

    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    

    Expression

    Bacterial

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid

    #175570

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Mutation

    XbaI site added upstream of U6 promotor. G to C …

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. SL2 GFP H2B

    Plasmid

    #227717

    Purpose

    Plasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

    Depositor

    Haosheng Sun, Oliver Hobert

    Article

    Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

    Insert

    SL2 GFP H2B (nlp-51 Nematode)
    

    Use

    Cloning vector

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-EOS-GFP-IP

    Plasmid

    #60439

    Purpose

    PiggyBac for EOS-GFP

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    EOS
    

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. NM1-5S-tRNA-SgH

    Plasmid

    #128178

    Purpose

    Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    gRNA cloning cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    5S rRNA-tRNA(Arg)

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSelAct-KO

    Plasmid

    #174374

    Purpose

    Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible

    Depositor

    Gitta Coaker

    Article

    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

    Type

    Empty backbone

    Use

    Vector for 2-step knockout by homologous recombin…

    Promoter

    N/A

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQIq_MRGS_HIS8_(27F3)_FLAG

    Plasmid

    #199615

    Purpose

    Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG 27F3
    

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKM495 (Flag-Das+4) Zeo

    Plasmid

    #109284

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with degradation tag DAS+4, using zeocin as a selection marker.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-Flag-DAS+4
    

    Use

    Orbit integrating plasmid

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBWB294

    Plasmid

    #140636

    Purpose

    pBAV1k-lacI-Trc-BCD9-mCherry. BCD RBS.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDD284

    Plasmid

    #66825

    Purpose

    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pRN1120

    Plasmid

    #101750

    Purpose

    Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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