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pLenti_CMV:Flag-LumiScarlet_SV40:PuroR

Plasmid

#183044

Purpose

Expresses FLAG-tagged LumiScarlet in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

LumiScarlet

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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LCMV:GFP(FRT)DsRed

Plasmid

#31378

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

GFP(FRT)DsRed

Use

Flp/frt

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-M2-TLR2-P681H

Plasmid

#12290

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

A74V, M82I, H202Y and P681H; signal peptide (aa1-…

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-myc-FRNK

Plasmid

#74481

Purpose

This plasmid was constructed in order to characterize the function of Proline-rich tyrosine kinase 2 (PYK2).

Depositor

J Thomas Parsons

Article

Xiong et al J Cell Biol. 1997 Oct 20;139(2):529-39.

Insert

FRNK (PTK2 Chicken)

Tags

c-myc

Expression

Mammalian

Mutation

FRNK is the C-terminal, noncatalytic domain of th…

Promoter

CMV

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-cAICDA-BE3

Plasmid

#113422

Purpose

Expresses cAICDA-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

cAICDA-BE3 (aicda Ictalurus punctatus (channel catfish))

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-Cx35-Halo-C-in

Plasmid

#71511

Purpose

Mammalian expression plasmid encoding perch Cx35 with an internal HaloTag between amino acids 285 and 286 in the C-terminus.

Depositor

John O'Brien

Article

Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

Insert

Connexin 35

Tags

HaloTag (internal to sequence)

Expression

Mammalian

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pBSHSP:FLP;CMV:tdTomato-SceI

Plasmid

#30523

Depositor

Gerhart Ryffel

Article

Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

Insert

FLP recombinase tdTomato

Use

Flp/frt, heat shock inducible, i-scei megabase ve…

Mutation

R415H

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-nTEVp

Plasmid

#179666

Purpose

Ras Binding Domain split-nTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

nTEVP

Expression

Mammalian

Mutation

K65E

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-cTEVp

Plasmid

#179667

Purpose

Ras Binding Domain split-cTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

cTEVP

Expression

Mammalian

Mutation

K65E

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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(E6) CMV-p18 C/EBPalpha

Plasmid

#25881

Depositor

Ormond MacDougald

Article

Erickson et al J Biol Chem. 2001 May 11. 276(19):16348-

Insert

p18 C/EBPalpha (Cebpa Mouse)

Expression

Mammalian

Mutation

N-terminal truncation, contains only CR4 and bZIP…

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
pCMV_SEPT2 NCmut-mApple

Plasmid

#180321

Purpose

mammalian expression of human SEPT2 NCmut fused to monomeric Apple

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

mApple

Expression

Mammalian

Mutation

SEPT2 F20D, V27D

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRRLsin.PPT.CMV‐GFP‐Eps8 WT

Plasmid

#74922

Purpose

Lentiviral plasmid expressing Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Menna et al PLoS Biol. 2009 Jun 30;7(6):e1000138. do

Insert

Eps8 (Eps8 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
CMV-TO-nTEVPtvhcZ-2A-ZhctvcTEVP

Plasmid

#116064

Purpose

Expresses an engineered TEV protease

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

nTEVPtvhcZ-2A-ZhctvcTEVP

Expression

Mammalian

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-2xFLAG-Atoh1ER

Plasmid

#101736

Purpose

Expresses mouse Atoh1 fused to ER

Depositor

Albert Edge

Article

Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

Insert

Atoh1 (Atoh1 Mouse)

Tags

2x FLAG and ER

Expression

Mammalian

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM3-K83,88,104A

Plasmid

#58405

Purpose

Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysines 83, 88, and 104 to Alanine

Promoter

CMV IE

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K83A

Plasmid

#58401

Purpose

Expresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysine 83 to Alanine

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24A

Plasmid

#58400

Purpose

Expresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysine 24 to Alanine

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-H-RAN-H2A2B

Plasmid

#106419

Purpose

H-RAN-H2A2B (RANbody H2A2B::Sm-HAtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

H-RAN-H2A2B

Tags

HA, His, and Multiple HA

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D1 C7G HA

Plasmid

#8954

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Mutation

C7G: point mutation in LxCxE motif, LxghE

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pCMV1-OCT-HA-eGFP

Plasmid

#67480

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Expression

Mammalian

Promoter

attenuated CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only