We narrowed to 11,411 results for: ENA
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Plasmid#119058PurposeFor expression in E.coli and affinity purification of N-terminally His tagged NQO1DepositorInsertNAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
Tags6His, TEV siteExpressionBacterialPromoterT7Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-Cas9-Act3.0
Plasmid#178954PurposeIt consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.DepositorInsertzCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRExpressionPlantAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
GPD-roGFP
Plasmid#49436PurposeExpresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cellsDepositorInsertGPD-roGFP2
UseAdenoviralTagsGlycerol phosphate dehydrogenase, 5' to roGF…ExpressionMammalianMutationC48S, Q80R, S147C, and Q204C in Clontech's G…PromoterCMVAvailable SinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
hEGFR-mEGFP
Plasmid#182878PurposeMammalian expression of epidermal growth factor receptor (EGFR) fused to mEGFPDepositorInserthEGFR-mEGFP (EGFR Human)
ExpressionMammalianAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCHC027 (∆pemK)
Plasmid#226204PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.DepositorInsertUpstream/downstream homology arms for addiction system toxinPemK.
UseSynthetic BiologyAvailable SinceNov. 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFlp-Km
Plasmid#222334PurposeExpresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3DepositorAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 NQO1-Flag
Plasmid#61729PurposeExpresses human NQO1 Flag tagged at C-terminusDepositorInsertNAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
TagsFlagExpressionMammalianPromotercmvAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAluYa5-neo-TET
Plasmid#51283Purposeexpresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertAluYa5
TagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianPromoterupstream pol III enhancer sequence of the 7SL gen…Available SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogCatcher-MBP
Plasmid#171928PurposeExpresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogCatcher-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationDogCatcher added to N-terminus of MBP via a GSGES…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCarCBAE
Plasmid#117755PurposeCodon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backboneDepositorInsertCodon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
ExpressionBacterialPromoterPLac(UV5)Available SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only