164,577 results

1. YL-CashGem-BAT1-URA3-LINEAR

   Plasmid

   #174837

   Purpose

   pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GPDp

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW-TetO-Klf5

   Plasmid

   #128830

   Purpose

   dox-dependent expression of mouse Klf5

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   Klf5 (Klf5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSilencer-Opa1 KO

   Plasmid

   #191949

   Purpose

   Expression of a siRNA that targets Opa1

   Depositor

   Luca Scorrano

   Insert

   Opa1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-puro/Flag PD-L1 N35/3NQ

   Plasmid

   #121455

   Purpose

   expression of human PD-L1 mutant in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N192Q, N200Q and N219Q

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRB051_LEU2

   Plasmid

   #196993

   Purpose

   Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

   Depositor

   Matthew Maurano

   Article

   Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEP203-Lenti-TRE3G-GFPGluN2B-WPRE-pA

   Plasmid

   #164787

   Purpose

   pLenti vector backbone designed to express GFP-GluN2B from a TRE3G promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2B
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   1.3αCaMKII

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PK187

   Plasmid

   #44623

   Depositor

   Robert Oshima

   Article

   Oshima et al Differentiation. 1986;33(1):61-8.

   Insert

   K18 (KRT18 Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1-human LIC1 C-terminal half

   Plasmid

   #74599

   Purpose

   used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
   

   Use

   Tags

   N-terminal GST and Strep II

   Expression

   Bacterial

   Mutation

   amino acids 389-523 of LIC1

   Promoter

   T7

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTYB2-eIF1A

   Plasmid

   #37218

   Depositor

   Jon Lorsch

   Article

   Acker et al Methods Enzymol. 2007;430:111-45.

   Insert

   eIF1A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

10. p404GALS

    Plasmid

    #17435

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    GALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    GALS promoter is between SacI and XbaI restrictio…

    Promoter

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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