Addgene

We narrowed to 9,766 results for: 158

Showing: 3211 - 3240 of 9766 results

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pcDNA3/Myc-DNMT3A2

Plasmid

#36941

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3A2 (DNMT3A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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HA-merlin

Plasmid

#32836

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Merlin (NF2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only
pcDNAHygro(+)LAMP2AS

Plasmid

#86146

Purpose

Anti sense for LAMP2

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

LAMP2a (LAMP2 Human)

Use

Tags

Expression

Mammalian

Mutation

contains first 525 bases of LAMP cDNA

Promoter

CMV

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P4:gs:nls:VPR

Plasmid

#135983

Purpose

Expresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the VPR activation domain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAF0443 Nm60_attP-mCherry donor

Plasmid

#193471

Purpose

Promoterless mcherry donor for Nm60

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Nm60_attP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK5 K232R

Plasmid

#80878

Purpose

mammalian expression of ALK5 K232R

Depositor

Aristidis Moustakas

Article

Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

Insert

ALK5 (TGFBR1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K232R (kinase dead)

Promoter

CMV

Available since

Aug. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1

Plasmid

#178603

Purpose

Mammalian expression of myc-tagged MR1

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Use

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAF0466 Ef1a-Nm60_attB acceptor

Plasmid

#193476

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Nm60_attB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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TDP43 NOTAG2

Plasmid

#28207

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

aa86-414 only

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
SaCas9 TadDE

Plasmid

#193846

Purpose

Express TadDE (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE with SaCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pJFRC-20XUAS-IVS-PhiC31

Plasmid

#133565

Purpose

Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

PhiC31 Recombinase (int )

Use

Tags

Expression

Insect

Mutation

Promoter

20X UAS

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_eCB2.0

Plasmid

#164609

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Tags

Expression

Mutation

S383T

Promoter

GfaABC1D

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMIR-His-Halo-Tev-PTEN

Plasmid

#58241

Purpose

expresses halo tagged PTEN protein

Depositor

Yimon Aye

Article

Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

Insert

phosphatase and tensin homolog (PTEN Human)

Use

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pEF1alpha-rtTA-Antares

Plasmid

#197587

Purpose

Expresses TET-On reverse transactivator labeled with Antares

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

rtTA-P2A-Antares

Use

Tags

Antares

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3B3

Plasmid

#37546

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3B3 (DNMT3B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pTRE3G-iRFP-B1-timestamp_A

Plasmid

#159924

Purpose

Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

iRFP-Timestamp A

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

Plasmid

#191145

Purpose

Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

3UTR(Bdnf)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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CREB3L3

Plasmid

#99509

Purpose

to express CREB3L3 in mammalian system

Depositor

Dong-Yan Jin

Article

Chin et al Nucleic Acids Res. 2005 Mar 30;33(6):185

Insert

CREB3L3 (Creb3l3 Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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eNme2-C TadDE

Plasmid

#193842

Purpose

Express TadDE (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

eNme2-C TadDE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3B2

Plasmid

#36942

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3B2 (DNMT3B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)

Plasmid

#191141

Purpose

Positive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codon

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587-W:EGFP

Use

Tags

Expression

Mammalian

Mutation

UAG>UGG (stop>Trp)

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pWay21-MKP-1 FL

Plasmid

#13469

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1 FL (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-Merlin S518A

Plasmid

#84294

Purpose

Expression of Merlin with an alanine mutation at the Pak phosphorylation site

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S518A

Promoter

CMV

Available since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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(112) pcDNA3.1-GFP-Flag-Nup62-EPEA

Plasmid

#168101

Purpose

GFP and Flag tagged Nup62 with EPEA nanobody tag

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

GFP-Flag-Nup62-EPEA

Use

Tags

EPEA, Flag, and GFP

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-SI-121

Plasmid

#131126

Purpose

A-to-G base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

mutant EGFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pRN110

Plasmid

#127228

Purpose

CmYLCV::STM::35S terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

STM (STM A. tumefaciens, Mustard Weed)

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Cry2L348F(1-531)-mCh-BAXS184E

Plasmid

#117239

Purpose

Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

Cry2531.L348F-mCh-BAXS184E

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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P55.SRE.mCMV.flag.mSrtA-3M.wpre

Plasmid

#226774

Purpose

secretory SortaseA in p53 (SRE backbone)

Depositor

Ferdinando Pucci

Article

Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

Insert

sortase A (srtA )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB6a

Plasmid

#19529

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB6a (DNAJB6 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-Merlin S518D

Plasmid

#84295

Purpose

Expression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation site

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S518D

Promoter

CMV

Available since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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