We narrowed to 10,180 results for: yeast

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pHHYTK127

Plasmid

#200139

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Fox2-PTS1 (870-900)

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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RS314-U6(PolII-Lsm)

Plasmid

#200772

Purpose

Pol II expression of U6 Lsm

Depositor

Aaron Hoskins

Article

Lipinski et al RNA. 2022 Dec;28(12):1606-1620. doi: 10.

Insert

Gal-SNR6-II

Expression

Bacterial and Yeast

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-leuYFP[CTT]-nLuc

Plasmid

#199757

Purpose

Elongation reporter construct to quantify elongation of synYFP[CTT]

Depositor

Brian Zid

Article

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

leuYFP[CTT]-nLuc

Expression

Yeast

Promoter

TetO7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-leuYFP[CTC]-nLuc

Plasmid

#199758

Purpose

Elongation reporter construct to quantify elongation of synYFP[CTC]

Depositor

Brian Zid

Article

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

leuYFP[CTC]

Expression

Yeast

Promoter

TetO7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK125

Plasmid

#200137

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

OM-Tom70 (2-98)

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK107

Plasmid

#200133

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Kar2-HDEL

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK126

Plasmid

#200138

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Pot1-PTS2 (2-50)

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK106

Plasmid

#200132

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Kar2-SS

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK124

Plasmid

#200136

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

IMS-Mia40 (2-70)

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK123

Plasmid

#200130

Purpose

Type 7 part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

pRS CEN/ARS

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17

Plasmid

#193319

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron). ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

IAA17

Expression

Yeast

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT Compatible pAIDA2(6FLAG)

Plasmid

#193321

Purpose

C-SWAT compatible vector for C-terminal tagging with TAP-AID*-6FLAG. osTIR1 is carried downstream of the C-terminal tag.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

TAP-AID*-6FLAG

Tags

TAP-AID*-6FLAG

Expression

Yeast

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT AID*-6MYC osTIR1

Plasmid

#193320

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Tags

6MYC

Expression

Yeast

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT AID*-6FLAG osTIR1

Plasmid

#182515

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Tags

6FLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-6FLAG

Plasmid

#182516

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

6FLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-3HA

Plasmid

#182517

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3HA. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

3HA

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-3MYC

Plasmid

#182518

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3MYC. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

3MYC

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SIR4 gRNA HIS

Plasmid

#182520

Purpose

Expression vector for gRNA directed against SIR4 ORF.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

SIR4 gRNA (SIR4 Budding Yeast)

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-6FLAG

Plasmid

#182522

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 with CAN1 homology arms for genome integration.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

6FLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2At(2nd)

Plasmid

#183085

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2At(2nd)

Plasmid

#183086

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEDJ8

Plasmid

#177271

Purpose

Double gRNA for RNH1 RNH201 knock-out

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Insert

gRNA_RNH1_RNH201

Expression

Bacterial and Yeast

Promoter

SNR52

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1 WD

Plasmid

#163580

Purpose

express Dcp2 ΔH1 WD at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

W50A, D54A, L255A

Promoter

DCP2 promoter

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1

Plasmid

#163576

Purpose

express Dcp2 ΔH1 (L255A) at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Lysine at position 255 is mutated to Alanine

Promoter

DCP2 promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJT9RFR

Plasmid

#165067

Purpose

Introducing GAL promoters-controlled sequiterpene nerolidol-producing module and Y-FAST and in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

T-RPL3>ERG20>P-GAL1-P-GAL2>Y-FAST>ErB1.2A-AcNES1>T-RPL41B

Expression

Yeast

Mutation

WT

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP1H5

Plasmid

#165065

Purpose

Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFPY2A

Plasmid

#165066

Purpose

Characterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFP

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>Y-FAST-ErB1.2A-yEGFP>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pISKP1-TIR1

Plasmid

#165061

Purpose

Integrative expression of SKP1-fusing rice auxin receptor gene OsTIR1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1- T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP105

Plasmid

#165060

Purpose

Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP104

Plasmid

#165059

Purpose

Integrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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