We narrowed to 3,857 results for: SPL;

1. AviTag-SpyTag003-MBP

   Plasmid

   #184227

   Purpose

   Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   AviTag-SpyTag003-MBP
   

   Use

   Tags

   AviTag, His6, SpyTag003, and Thrombin cleavage si…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. OPA1-isoform7

   Plasmid

   #70179

   Purpose

   Mammalian expression of hsOPA1 isoform 7

   Depositor

   David Chan

   Article

   Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

   Insert

   hsOPA1 isoform 7 (OPA1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. FlagETR3 (CELF2)

   Plasmid

   #96900

   Purpose

   expression of Flag-tagged human CELF2 (aka CUGBP2)

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   human CELF2 cDNA (CELF2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYSD099

   Plasmid

   #212923

   Purpose

   A MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTrcHisB-TRF2

   Plasmid

   #50488

   Purpose

   E. Coli N-term HexHis Tagged Protein Expression Vector

   Depositor

   Jack Griffith

   Article

   Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

   Insert

   TRF2 (TERF2 Human)
   

   Use

   Tags

   6x His, T7 tag, and TEV protease site

   Expression

   Bacterial

   Mutation

   Contains amino acids 3-500

   Promoter

   pTrc

   Available since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT9624-BEAR-cloning

   Plasmid

   #162986

   Purpose

   BEAR target cloning plasmid with split EGFP

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-3xFLAG-Rbfox3

   Plasmid

   #213727

   Purpose

   Mammalian cell expression vector for overexpression of Rbfox3 in motor neurons.

   Depositor

   Chaolin Zhang

   Article

   Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

   Insert

   Rbfox3 (Rbfox3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. OPA1-isoform5

   Plasmid

   #70177

   Purpose

   Mammalian expression of hsOPA1 isoform 5

   Depositor

   David Chan

   Article

   Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

   Insert

   hsOPA1 isoform 5 (OPA1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGGS-3xFLAG-Rbfox1

   Plasmid

   #213725

   Purpose

   Mammalian cell expression vector for overexpression of Rbfox1 in motor neurons.

   Depositor

   Chaolin Zhang

   Article

   Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

   Insert

   Rbfox1 (Rbfox1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-Cas13d-T2A-GFP(1-10)

    Plasmid

    #224566

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-GFP(1-10)
    

    Use

    CRISPR

    Tags

    T2A-split GFP(1-10)

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-nucGFP11-3xControlgRNA

    Plasmid

    #224568

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. OPA1-isoform2

    Plasmid

    #70174

    Purpose

    Mammalian expression of hsOPA1 isoform 2

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 2 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. OPA1-isoform3

    Plasmid

    #70175

    Purpose

    Mammalian expression of hsOPA1 isoform 3

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 3 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

14. OPA1-isoform4

    Plasmid

    #70176

    Purpose

    Mammalian expression of hsOPA1 isoform 4

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 4 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

15. SMN2E7-Fluc-Nluc-reporter

    Plasmid

    #221005

    Purpose

    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    Fluc-SMN2E7-STOP-Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. OPA1-isoform6

    Plasmid

    #70178

    Purpose

    Mammalian expression of hsOPA1 isoform 6

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 6 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-hNox1

    Plasmid

    #58344

    Purpose

    expresses human Nox1 in mammalian cells

    Depositor

    Karl-Heinz Krause, Botond Banfi

    Article

    Banfi et al Science. 2000 Jan 7;287(5450):138-42.

    Insert

    NADPH oxidase 1 (NOX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. FlagMBNL1-41

    Plasmid

    #96906

    Purpose

    expresses Flag tagged human MBNL1-41 kDa isoform from pcDNA3.1 HisC

    Depositor

    Thomas Cooper

    Article

    Grammatikakis et al Nucleic Acids Res. 2011 Apr;39(7):2769-8

    Insert

    MBNL1-41 kDa isoform (MBNL1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV7.1_3xFLAG-WWC3

    Plasmid

    #172996

    Purpose

    constitutive expression of FLAG-tagged WWC3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-WWC3 (WWC3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGLOW77

    Plasmid

    #177338

    Purpose

    C. elegans mNeonGreen co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-2::mNeonGreen
    

    Use

    Tags

    mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    myo-2

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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