We narrowed to 2,685 results for: buc

1. pCRIS-PITChv2-dTAG-BSD (BRD4)

   Plasmid

   #91795

   Purpose

   PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

   Depositor

   Behnam Nabet, James Bradner

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   FKBP_F36V-2xHA-P2A-BSD
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRIS-PITChv2-BSD-dTAG (BRD4)

   Plasmid

   #91792

   Purpose

   PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   Behnam Nabet, James Bradner

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   BSD-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promoterless

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CL20_mEGFP_TCF3_PBX1

   Plasmid

   #205937

   Purpose

   Express mEGFP-tagged fusion protein, TCF3_PBX1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   TCF3_PBX1 (TCF3, PBX1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH1-CMV-HT-LC3-SV40-Hygro

   Plasmid

   #182045

   Purpose

   Lentiviral expression vector encoding HaloTag-LC3 for monitoring autophagosome closure

   Depositor

   Hong-Gang Wang

   Article

   Takahashi et al J Cell Biol. 2019 Oct 7;218(10):3336-335

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   HaloTag-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pESF140_HisSpy_DDB1∆B

   Plasmid

   #124213

   Purpose

   Insect cell expression vector for His6, Spy tagged hsDDB1∆B

   Depositor

   Eric Fischer

   Article

   Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

   Insert

   DNA damage-binding protein 1 (DDB1 Human)
   

   Use

   Tags

   His6-Spy

   Expression

   Insect

   Mutation

   internal deletion of BPB domain to aid crystalliz…

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

   Plasmid

   #207805

   Purpose

   E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

   Depositor

   Koen Breyne

   Article

   Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

   Insert

   E-NoMi
   

   Use

   Lentiviral

   Tags

   3xFlag tag, copGFP, mCherry, and nanoluciferase

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6.2_3xFLAG_V5_ mmDdx58_V5

   Plasmid

   #160108

   Purpose

   Expresses murine Ddx58 in mammalian cells

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   Ddx58 (Rigi Mouse)
   

   Use

   Lentiviral

   Tags

   3xFLAG, V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CL20_mEGFP_NCOA4_RET

   Plasmid

   #205865

   Purpose

   Express mEGFP-tagged fusion protein, NCOA4_RET from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   NCOA4_RET (NCOA4, RET Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHLS-EF1a-FKBP12-Gag(HIV)

   Plasmid

   #138476

   Purpose

   Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   HIV Gag
   

   Use

   Tags

   FRBP12 and Lynn Myristolation site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PL-5LTR-RGR(DMD​#​1​)-AmCyan-A

    Plasmid

    #138482

    Purpose

    Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Ribozyme-flanked gRNA and AmCyan
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid

    #181744

    Purpose

    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    bacterial codon optimized SpRY-HF1
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS(SV40)-6xHis and MKIEE MBP tag

    Expression

    Bacterial

    Mutation

    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…

    Promoter

    T7

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMHE-mScarlet-MAP4-MTBD

    Plasmid

    #171488

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    MAP4-MTBD (Map4 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Expression

    Mutation

    Aminoacids 658-1126

    Promoter

    T7

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEMHE-miRFP-MAP4-MTBD

    Plasmid

    #171487

    Purpose

    T7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    MAP4-MTBD (Map4 Mouse)
    

    Use

    Pgehme

    Tags

    miRFP

    Expression

    Mutation

    Aminoacids 658-1126

    Promoter

    T7

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CL20_mEGFP_MYC_MYC

    Plasmid

    #205862

    Purpose

    Express mEGFP-tagged fusion protein, MYC_MYC from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    MYC_MYC (MYC Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CL20_mEGFP_TANC2_CRB3

    Plasmid

    #205933

    Purpose

    Express mEGFP-tagged fusion protein, TANC2_CRB3 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    TANC2_CRB3 (TANC2, CRB3 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff

    Plasmid

    #107700

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    QuasAr3-P2A-CheRiff
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMSCV-IRES-GFP/CALR del52-FLAG

    Plasmid

    #204523

    Purpose

    Retroviral expression of human CALR del52-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR del52-FLAG (CALR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    del52

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-IRES-GFP/CALR ins5-FLAG

    Plasmid

    #204524

    Purpose

    Retroviral expression of human CALR ins5-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR ins5-FLAG (CALR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    ins5

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CL20_mEGFP_PTEN_RPL11

    Plasmid

    #205903

    Purpose

    Express mEGFP-tagged fusion protein, PTEN_RPL11 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    PTEN_RPL11 (PTEN, RPL11 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR223.1_mmDdx58 (closed)

    Plasmid

    #160107

    Purpose

    Expresses murine Ddx58 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ddx58 (Rigi Mouse)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge