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pDCB014

Plasmid

#135433

Purpose

Yeast integrating plasmid to express MCP-ABI and PYL1-VP64

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Inserts

MCP-ABI
PYL1-VP64

Expression

Yeast

Promoter

pAdh1 and pHHF2

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Rac1

Plasmid

#163607

Purpose

Expresses Rac1 Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Rac1 (Rac1 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSB4A5 JQ929582

Plasmid

#38169

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

mKate2
Superfolder GFP

Use

Synthetic Biology; Biobrick cloning vector

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pCJH031_proD_LbCas12a-gRNA-GFP_CAM

Plasmid

#183075

Purpose

Plasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA targeting GFP

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-ZifCY1

Plasmid

#36315

Depositor

Amy Palmer

Article

Dittmer et al J Biol Chem. 2009 Jun 12;284(24):16289-9

Insert

ZifCY1 genetically encoded Zn(II) sensor

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only
YCplac111-yVps23-mCherry

Plasmid

#45925

Depositor

James Hurley

Article

Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

Insert

yeast Vps23 (STP22 Budding Yeast)

Tags

mCherry

Expression

Yeast

Promoter

lac

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
SI11-Sema4c

Plasmid

#46301

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Sema4c mouse monoclonal antibody (sc:d411 Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
p150 pPr-beta-gal

Plasmid

#8402

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

probasin promoter (Pbsn Rat)

Expression

Mammalian

Available Since

Dec. 29, 2005

Availability

Academic Institutions and Nonprofits only
M71::GFP LTNL TV

Plasmid

#15618

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

M71::GFP LTNL TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pBad_crtEBI_27

Plasmid

#63806

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1(pLtetO-TP901-aav-proA-tetR)-katGp-PhiC31-proD-oxyR

Plasmid

#78228

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only
pOX + kqt2

Plasmid

#16137

Depositor

Larry Salkoff

Article

Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

Insert

Caenorhabditis elegans potassium channel (kqt-2 Nematode)

Use

Xenopus oocyte expression

Mutation

Deletion of aa1-38 compared to GenBank NP_509392.3

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
GST cdc25c C330S C377S

Plasmid

#10970

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c C330S C377S (CDC25C Human)

Tags

GST

Expression

Bacterial

Mutation

C330S C377S. Cysteine to Serine substitution at …

Available Since

Nov. 23, 2005

Availability

Academic Institutions and Nonprofits only
pET15b/GgVcl 884-1066 (aka Vt)

Plasmid

#46176

Depositor

Susan Craig

Article

Steimle et al J Biol Chem. 1999 Jun 25;274(26):18414-2

Insert

Vcl 884-1066 (VCL Chicken)

Tags

His

Expression

Bacterial

Mutation

aa 884-1066 (tail domain)

Promoter

T7

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pME18S-F-R-Olfr644

Plasmid

#149365

Purpose

Expresses Olfr644 in mammalian cells.

Depositor

Takeshi Imai

Article

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Insert

Olfr644 (Olfr644 Mouse)

Tags

FLAG and rhodopsin

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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p209 pCMV-cre-K

Plasmid

#8394

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

Modified 5' Cre

Use

Cre/Lox

Expression

Mammalian

Mutation

Modified 5' region of Cre

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pET28a-Hisx6-Sirt6-S56A

Plasmid

#20278

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Tags

Hisx6

Expression

Bacterial

Mutation

Serine 56 to Alanine

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
pSL6 - Brr2 del247 WT

Plasmid

#90256

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRB38

Plasmid

#72864

Purpose

template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

Strep - His8 - Strep -TEV tag

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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AlphaE

Plasmid

#124527

Purpose

pColE1_AlphaE

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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