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Showing: 4321 - 4360 of 8321 results

  1. pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53205
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and kanMX6
    Expression
    Mutation
    Promoter
    Gal1 promoter
    Available since
    May 27, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. PTPN5

    Plasmid
    #38887
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN5 (PTPN5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C496S inactivating mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. pT7-7 asyn S87A/S129E

    Plasmid
    #36065
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S87A/S129E (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    S87A/S129E
    Promoter
    T7
    Available since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    36065-plasmid-map-sequence-id-44522

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  4. pKG212

    Plasmid
    #69758
    Purpose
    Retroviral expression of CENP-T aa 1-242
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-242 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pVP16-tev-PRT1

    Plasmid
    #83405
    Purpose
    bacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83405-plasmid-map-sequence-id-175081

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  6. SDCBPA-c010

    Plasmid
    #175482
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175482-plasmid-map-sequence-id-353168

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  7. RHOU

    Plasmid
    #38885
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RHOU (RHOU Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C191L
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pFLAG-6a-Ataxin3Q22-UIM*

    Plasmid
    #22128
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    UIM 1,2,3 SA-DG
    Promoter
    Available since
    Nov. 17, 2009
    Availability
    Academic Institutions and Nonprofits only

  9. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid
    #121171
    Purpose
    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    xFNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121171-plasmid-map-sequence-id-235381

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  10. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

    Plasmid
    #121175
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121175-plasmid-map-sequence-id-235290

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  11. pUASp YFP Cdc42 WT

    Plasmid
    #52248
    Purpose
    Drosophila expression of YFP-Cdc42
    Depositor
    Matthew Scott
    Insert
    Cdc42 (Cdc42 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. FABP9

    Plasmid
    #39107
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FABP9 (FABP9 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pNtCp-rbcL-PHLS-aadA-accD

    Plasmid
    #52470
    Purpose
    Inserts the beta-phellandrene synthase gene and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum
    Depositor
    Anastasios Melis
    Insert
    rbcL-PHLS-aadA-accD
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52470-plasmid-map-sequence-id-81333

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  14. 4QQN

    Plasmid
    #61951
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HRMT1L3:APC035-H01:C21507 (PRMT3 Human)
    Use
    Tags
    6xHis-Thrombin and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. PTGR2

    Plasmid
    #38949
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTGR2 (PTGR2 Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pLV-Enh eGFP Reporter-muKC2-IGR13

    Plasmid
    #59282
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    17kb upstream krt5
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pCB99

    Plasmid
    #69745
    Purpose
    Retroviral expression of Centrin 2
    Depositor
    Iain Cheeseman
    Insert
    Centrin 2 (CETN2 Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pJK122

    Plasmid
    #74295
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusion
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Use
    Tags
    NusA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74295-plasmid-map-sequence-id-141881

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  19. mEos4a-CenpA-C-22

    Plasmid
    #62772
    Purpose
    Localization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581
    Depositor
    Loren Looger, Michael Davidson
    Insert
    CenpA (CENPA Human)
    Use
    Tags
    mEos4a
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pDK713

    Plasmid
    #134229
    Purpose
    Mammalian expresion of tdTomato SKAP
    Depositor
    Iain Cheeseman
    Insert
    DK360 tdTomato (long SKAP) (KNSTRN Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    134229-plasmid-map-sequence-id-272275

    Enlarge

  21. AKR7A3

    Plasmid
    #38862
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AKR7A3 (Akr7a3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. 4JDS

    Plasmid
    #60081
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD7:DCC011-H01:C212434 (SETD7 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pCRII-Topo Couptf1

    Plasmid
    #45870
    Depositor
    Jeffrey Macklis
    Insert
    Couptf1 in situ probe (Nr2f1 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    contains bp# 1755-2381 of NM_010151.2
    Promoter
    Available since
    July 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. pLV-Enh eGFP Reporter-muKC2-X1

    Plasmid
    #59200
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    krt1-ex1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. PRKCG

    Plasmid
    #38938
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PRKCG (PRKCG Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. pJK237

    Plasmid
    #73339
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase with cleavable N-terminal His6/CBD/Ssp-intein fusion
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6, Ssp intein, and chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73339-plasmid-map-sequence-id-138207

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  28. 4QQ4

    Plasmid
    #61945
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MORC3:JMC01B-H03:C203041 (MORC3 Human)
    Use
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. VIPR2

    Plasmid
    #39044
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    VIPR2 (VIPR2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. CS2-H2B-EGFP

    Plasmid
    #132414
    Purpose
    To make synthetic mRNA encoding a nuclear EGFP
    Depositor
    David Kimelman
    Insert
    H2B-EGFP (H2BC21 Synthetic)
    Use
    Synthetic mrna production
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    132414-plasmid-map-sequence-id-257700

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  31. pCDNA3-hPXR-FL-T248E

    Plasmid
    #41617
    Depositor
    Sridhar Mani
    Insert
    hPXR-FL-T248E (NR1I2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N10D and T248E
    Promoter
    CMV
    Available since
    March 11, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. mEos4a-CenpB-N-22

    Plasmid
    #62773
    Purpose
    Localization: Nucleus/Centromeres, N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581
    Depositor
    Loren Looger, Michael Davidson
    Insert
    CenpB (CENPB Human)
    Use
    Tags
    mEos4a
    Expression
    Mammalian
    Mutation
    Encodes amino acids 1-180
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pLV-Enh eGFP Reporter-muKC2-X39

    Plasmid
    #59263
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Krt83-ex5-7
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. Myc-Foxo1 LAALL (pCMV5)

    Plasmid
    #17548
    Depositor
    Domenico Accili
    Insert
    Foxo1 LAALL (Foxo1 Mouse)
    Use
    RNAi
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    LAALL (K460A; E461A)
    Promoter
    CMV
    Available since
    Oct. 8, 2008
    Availability
    Academic Institutions and Nonprofits only

  35. pZC10 (pFA6a-PSc-6xGly-V5-His3MX)

    Plasmid
    #44067
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44067-plasmid-map-sequence-id-61693

    Enlarge

  36. pTF266 (pFA6a-PSc-6xGly-3xFlag-His3MX)

    Plasmid
    #44065
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44065-plasmid-map-sequence-id-61669

    Enlarge

  37. pCRII-Topo Hnrp

    Plasmid
    #45872
    Depositor
    Jeffrey Macklis
    Insert
    Hnrnpa2b1 in situ probe (Hnrpa2b1 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    contains bp# 1145-1370 of NM_182650.3
    Promoter
    Available since
    July 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. pAc5.1B-EGFP-DmMTR-4

    Plasmid
    #66591
    Purpose
    expresses EGFP-tagged MTR-4 in Drosophila cells
    Depositor
    Elisa Izaurralde
    Insert
    MTR-4 (l(2)35Df Fly)
    Use
    Drosophila expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Actin 5C
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66591-plasmid-map-sequence-id-113866

    Enlarge

  39. pLV-Enh eGFP Reporter-muKC2-X33

    Plasmid
    #59257
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Krt81-ex4-7
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53207
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and HIS3MX6
    Expression
    Mutation
    Promoter
    GAL1 promoter
    Available since
    May 27, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 4321 - 4360 of 8321 results