We narrowed to 4,373 results for: AR

1. TIGRE docking site targeting vector

   Plasmid

   #61580

   Purpose

   Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET01-TAF5-ex8-Minigene-SRSF1 motif WT

   Plasmid

   #218972

   Purpose

   Vector for constitutive expression of TAF5 alternative exon-8 mini gene with wild type SRSF1 motif (TCAGAGGA)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 exon-8 (with wild type SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV LTR

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA6-N-3XFLAG-Ctnnb1

   Plasmid

   #123586

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   catenin (cadherin associated protein), beta 1 (Ctnnb1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT/TO-3*Flag-APEX2 N-term FBL

   Plasmid

   #187577

   Purpose

   Express FLAG epitope and APEX2-tagged FBL fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   FBL (FBL Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA6-N-3XFLAG-Dvl2

   Plasmid

   #123587

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   dishevelled segment polarity protein 2 (Dvl2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-puro-N-3HA-SREBP2-C-Flag

   Plasmid

   #134287

   Purpose

   Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP2 (Srebf2 Mouse)
   

   Use

   Lentiviral

   Tags

   3x HA and Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP2 precursor

   Promoter

   CMV

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT/TO-3*Flag-APEX2 N-term COILIN

   Plasmid

   #187581

   Purpose

   Express FLAG epitope and APEX2-tagged COILIN fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   COILIN (COIL Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-opCas12a-6xNLS

   Plasmid

   #209022

   Purpose

   Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   opCas12a-6xNLS-Flag-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and MYC

   Expression

   Mammalian

   Mutation

   E174R/S542R

   Promoter

   EF-1a

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

   Plasmid

   #65458

   Purpose

   Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   tTA2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1

    Plasmid

    #187586

    Purpose

    Express FLAG epitope and APEX2-tagged EWSR1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUDP002

    Plasmid

    #103872

    Purpose

    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Inserts

    hph
    Spcas9 D147Y P411T
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Yeast

    Mutation

    D147Y P411T

    Promoter

    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-Mito-Car-GECO

    Plasmid

    #100765

    Purpose

    Lentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    Lenti-Mito-Car-GECO
    

    Use

    Lentiviral

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    R-GECO1: E163V/I166V/V174T/M176I/F222I/A302P

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA6-N-3XFLAG-Lrp6

    Plasmid

    #123595

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    low density lipoprotein receptor-related protein 6 (Lrp6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Human 3' HP1a AID GFP PuroR

    Plasmid

    #127906

    Purpose

    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    HP1 (CBX5 Human, Mustard Weed)
    

    Use

    Donor plasmid

    Tags

    GFP, AID, PuroR

    Expression

    Mutation

    Inserting GFP AID 2A Puro into mouse 3' Hp1a

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

    Plasmid

    #208038

    Purpose

    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    PML (isoform IVa) (PML Human)
    

    Use

    Lentiviral

    Tags

    FLAG, N-terminal Split-TurboID

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD

    Plasmid

    #225702

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (K27M) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    K27M

    Promoter

    pEF1A

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-nSpCas9-NoABE

    Plasmid

    #209043

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid

    #222497

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSUMO3

    Plasmid

    #52260

    Purpose

    Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-3 (SUMO3 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ad:2CMV_A2R2-N25K3

    Plasmid

    #122242

    Purpose

    Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

    Depositor

    Corey Neu

    Article

    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

    Inserts

    C-terminal end of Nesprin-3 (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    

    Use

    Adenoviral

    Tags

    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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