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pLV-EF1alpha-DEST-IRES-Hygro

Plasmid

#253767

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to hygromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Milan R. et al Nature Metabolism, 29 April 2026

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-EF1alpha-DEST-IRES-Neo

Plasmid

#253768

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Milan R. et al Nature Metabolism, 29 April 2026

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-TRE-DEST-UbC-tTSrtTA-IRES-Puro

Plasmid

#253781

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a Tet-On inducible promoter for expression of a gene of interest linked to puromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Milan R. et al Nature Metabolism, 29 April 2026

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1alpha-DEST-IRES-Neo

Plasmid

#253766

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Milan R. et al Nature Metabolism, 29 April 2026

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-hPGK-DEST-IRES-Hygro

Plasmid

#253771

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a hPGK promoter for expression of a gene of interest linked to hygromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Milan R. et al Nature Metabolism, 29 April 2026

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pHimar6

Plasmid

#137081

Purpose

bacterial Himar1 mini-transposon donor plasmid

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar1 mini-transposon with chloramphenicol resistance gene

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-STRA8

Plasmid

#222585

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of STRA8, with hygromycin resistance

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

STRA8 (STRA8 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-pHlash

Plasmid

#61552

Purpose

Expression of pHlash in mammalian cells

Depositor

Carl Johnson

Article

Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

Insert

pHlash

Tags

NA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
pLV.CMV.GFPmiR125sponge

Plasmid

#115974

Purpose

Knockdown of miR-125

Depositor

Johan Jakobsson

Article

Akerblom et al Development. 2014 Apr;141(7):1580-8. doi

Insert

GFPmiR125sponge

Use

Lentiviral

Tags

GFP

Promoter

CMV

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pWJPrP43

Plasmid

#22112

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

PrP ki-3F4-FFI TC

Use

Mouse Targeting; Construction of targeting vector

Mutation

3F4 epitope and FFI related D177N mutation

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
Intersectin SH3 domain A

Plasmid

#47396

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain A

Tags

GST

Expression

Bacterial

Mutation

SH3 domain A

Promoter

tac

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
Intersectin SH3 domain B

Plasmid

#47397

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain B

Tags

GST

Expression

Bacterial

Mutation

SH3 domain B

Promoter

tac

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
Intersectin SH3 domain C

Plasmid

#47398

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain C

Tags

GST

Expression

Bacterial

Mutation

SH3 domain C

Promoter

tac

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
Intersectin SH3 domain E

Plasmid

#47400

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain E

Tags

GST

Expression

Bacterial

Mutation

SH3 domain E

Promoter

tac

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pCRII HSPB12

Plasmid

#61037

Purpose

Probe for HSPB12 for in situ hybridization in zebrafish

Depositor

Lara Hutson

Article

Marvin et al Dev Dyn. 2008 Feb;237(2):454-63.

Insert

HSPB12 (hspb12 Zebrafish)

Expression

Bacterial

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pWJPrP31

Plasmid

#22105

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

PrP exon 3 EcoR1 fragment

Use

Construction of targeting vector

Mutation

genomic EcoR1 fragment containing exon 3 of PrP

Available Since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only
pDonor_Tn[himar1C9]

Plasmid

#213906

Purpose

Mariner transposon with ATc-inducible himar1C9 transposase

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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CMV500 empty vector

Plasmid

#33348

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only

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CMV566 empty vector

Plasmid

#33349

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCB6 ZO1myc

Plasmid

#30317

Depositor

James Anderson, Alan Fanning

Article

Fanning et al J Biol Chem. 1998 Nov 6. 273(45):29745-5

Insert

Zonula Occludens-1 (TJP1 Human)

Tags

myc

Expression

Mammalian

Mutation

none

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only