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pDONR221 NLRP1 A54T

Plasmid

#166815

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

A54T

Promoter

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C NLRP1 UPA WT

Plasmid

#164010

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-URA3

Plasmid

#122894

Purpose

TRP1-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-HphMx

Plasmid

#122901

Purpose

TRP1-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-Drp1(deltaIB)

Plasmid

#73041

Purpose

Bacterial expression of mouse Drp1 isoform b (minus residues 514-602) with C-terminal His-tag and PreScission protease cleavage site

Depositor

David Chan

Article

Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

Insert

Drp1(deltaIB) (Dnm1l Mouse)

Use

Tags

6xHis-tag and PreScission Protease site

Expression

Bacterial

Mutation

Deleted amino acids 514-602

Promoter

T7

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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PYDonly-NLRP11.MYC

Plasmid

#131198

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

PYD domain of NLRP11 (NLRP11 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

contains only the pyrin domain (amino acids 1-128)

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-BIO-TRP1

Plasmid

#26881

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

BIO-tag

Use

Pcr tagging yeast

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-RGS18HIS-TRP1

Plasmid

#26884

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

18xHis tag

Use

Pcr tagging yeast

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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T7-His-Arp11(1–417)

Plasmid

#51400

Purpose

expression of full-length mouse Arp11 under T7 promoter

Depositor

Trina Schroer

Article

Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

Insert

Arp11 (Actr10 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pRS424 Prp8 WT TRP1

Plasmid

#234120

Purpose

The N-terminus of Prp8 was restored thereby destroying the NheI site and correcting the ORF

Depositor

Aaron Hoskins

Insert

Prp8

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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PB-PARP1-del.p119K120S–eGFP

Plasmid

#211554

Purpose

A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Piggybac

Tags

eGFP

Expression

Mammalian

Mutation

deletion of amino acids 119K 120S

Promoter

CMV

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0520 (pDEST, TRP1)

Plasmid

#210481

Purpose

pDEST vector with TRP1 marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Destination vector for gateway cloning

Tags

Expression

Mutation

Promoter

ADH1

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hARFRP1(T31N)-HA

Plasmid

#206401

Purpose

Mammalian expression of hARFRP1(T31N)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

Insert

ARFRP1 (ARFRP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T31N

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hARFRP1(Q79L)-HA

Plasmid

#206400

Purpose

Mammalian expression of hARFRP1(Q79L)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

Insert

ARFRP1 (ARFRP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Q79L

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET3-ARFRP1-His6

Plasmid

#201882

Purpose

Used for bacterial expression of human protein ARFRP1

Depositor

Richard Kahn

Insert

ARFRP1 (ARFRP1 Human)

Use

Tags

6xHis (GSGSGSHHHHHH)

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSCM167.2-Venus-VF-hSFRP1

Plasmid

#189541

Purpose

Protein expression of SFRP1 in mammalian cells.

Depositor

Haian Fu

Insert

secreted frizzled related protein 1 (SFRP1 Human)

Use

Tags

VF

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgLrp1b#2/Cre

Plasmid

#173642

Purpose

Expresses a Lrp1b-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Lrp1b (Lrp1b Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgLrp1b#1/Cre

Plasmid

#173641

Purpose

Expresses a Lrp1b-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Lrp1b (Lrp1b Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn1-L713F

Plasmid

#173937

Purpose

Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn1-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 1-96; L713F, V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn3-L713F

Plasmid

#173934

Purpose

Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; L713F, V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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