1,878 results

1. pJK2020

   Plasmid

   #171932

   Purpose

   express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon

   Depositor

   Judith Kimble

   Article

   Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

   Insert

   Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
   

   Tags

   superfolder GFP, mCherry

   Expression

   Worm

   Promoter

   -

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXF89

   Plasmid

   #139030

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   halotag (no STOP codon, PATC introns)
   

   Expression

   Worm

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEY41

   Plasmid

   #191045

   Purpose

   dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   dat-1 (dat-1 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEY43

   Plasmid

   #191047

   Purpose

   eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   eat-4(prom6-1) (eat-4 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ1320

   Plasmid

   #84829

   Purpose

   Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized GFP
   

   Expression

   Bacterial and Worm

   Promoter

   Peft-3

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Prab-3::NLS::GCaMP6s

   Plasmid

   #68119

   Purpose

   Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

   Depositor

   Andrew Leifer

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

   Insert

   rab-3::NLS::GCaMP6s
   

   Expression

   Worm

   Promoter

   rab-3

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK2014

   Plasmid

   #171931

   Purpose

   To express sygl-1 fused to 24 copies of MS2 loops.

   Depositor

   Judith Kimble

   Article

   Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

   Insert

   Psygl-1::24XMS2 loops::3X Flag::sygl-1 ORF::sygl-1 3'UTR
   

   Tags

   N-terminal Tag 24X MS2 loops

   Expression

   Worm

   Promoter

   -

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS3-rig-3P::AI::lgc-47::SL2::GFP::unc-54-3'UTR

   Plasmid

   #225924

   Purpose

   Expressing LGC-47 in AVA neuron

   Depositor

   Andrew Leifer

   Article

   Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

   Insert

   LGC-47 (lgc-47 Nematode)
   

   Expression

   Worm

   Promoter

   rig-3

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEM255 - sgRNA mosTI Pmlc-2_GFP

   Plasmid

   #159835

   Purpose

   Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI Pmlc-2_GFP
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJA42

    Plasmid

    #59930

    Purpose

    gRNA for cleavage at rol-6(su1006) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    rol-6(su1006) gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCM1.36

    Plasmid

    #17249

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    tbb-2 3'UTR in pDONRP2RP3
    

    Use

    Gateway

    Available Since

    Feb. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ910

    Plasmid

    #44481

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, NeoR, MCS
    

    Use

    Multiple cloning site vector

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. JH02

    Plasmid

    #73511

    Purpose

    mCherryo_PH pCFJ150 - contians C. elegans sequence optimized mCherry

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::mCherryo::PH::tbb-2
    

    Tags

    mCherry

    Promoter

    pmex-5

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFS16 Pmyo-3 NPP-9 mChBioFLAG

    Plasmid

    #45099

    Depositor

    Steven Henikoff

    Article

    Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

    Inserts

    npp-9 (npp-9 Nematode)
    unc-119 (unc-119 Nematode)
    

    Tags

    BLRP, FLAG, and mCherry

    Expression

    Worm

    Promoter

    myo-3 and unc-119

    Available Since

    June 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGLOW77

    Plasmid

    #177338

    Purpose

    C. elegans mNeonGreen co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-2::mNeonGreen
    

    Tags

    mNeonGreen

    Expression

    Worm

    Promoter

    myo-2

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEG345

    Plasmid

    #40077

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (mex-5 Nematode)
    

    Tags

    Dendra2 PFAF, S-peptide, and TEV protease cleavag…

    Expression

    Worm

    Promoter

    pie-1

    Available Since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCZGY2850

    Plasmid

    #87758

    Purpose

    Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pcol-19-PH-miniSOG(Q103L)
    

    Tags

    PH

    Expression

    Worm

    Mutation

    Q103L

    Promoter

    Pcol-19

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

    Plasmid

    #159853

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mNeon(PATC)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. KG#62

    Plasmid

    #110874

    Purpose

    Expresses the C. elegans acy-1 cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

    Inserts

    rab-3 promoter
    acy-1 cDNA
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. TC358

    Plasmid

    #122613

    Purpose

    TRE-delta-pes-10-GFP

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    7XTRE-delta-pes-10-GFP
    

    Expression

    Worm

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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