We narrowed to 387 results for: Amh
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Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
ExpressionBacterialPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHKMC1: Empty sgRNA for Cloning
Plasmid#67720PurposeEmpty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.DepositorTypeEmpty backboneExpressionWormPromoterPU6Available SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
7iBi89 (rat fibronectin)
Plasmid#14065DepositorInsertRat Fibronectin 7iBi89 (Fn1 Rat)
ExpressionMammalianMutationInsert was cut out of pGEM3 with SalI and SacI an…Available SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
MXS_MCS1
Plasmid#62395PurposeMXS_chaining vector with MCS1DepositorInsertEcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS
UseSynthetic BiologyAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) HIS-tag pET
Plasmid#8492DepositorInsertHoxA9 (Hoxa9 Mouse)
TagsHis and T7ExpressionBacterialMutationBamHI/BalI fusion into amino acid #2 of HOXA9; ma…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF71
Plasmid#185860PurposeTesting the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B9)
Plasmid#185858PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72
Plasmid#185857PurposeTesting the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1B
Plasmid#185856PurposeTesting the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1A
Plasmid#185855PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG-Nde-Acc
Plasmid#33150DepositorInsertRP51A-synthetic minimal intron (RPS17A Budding Yeast)
TagsLacZExpressionYeastMutationAccI site in intron was cut/filled/ligated to cha…Available SinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFU-GW-sMYH6-mNeon-TNNT2-mScarlet
Plasmid#170712Purposedual fluorescent reporter for MYH6 (short) and TNNT2 promotersDepositorInsertsmNeonGreen
mScarlet
UseLentiviralExpressionMammalianPromoterTNNT2 and aMHCAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (S939A/T1462A)
Plasmid#14131DepositorAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
9X NFAT-apha-MHC-Luc
Plasmid#51941Purpose9 copies of NFAT sites in minimal alphaMHC-pGL-3DepositorInserts9x NFAT binding site
alpha-MHC
UseLuciferaseTagsluciferaseAvailable SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HA-GAAZFP-FokI-RR
Plasmid#18754DepositorInsertzinc finger nuclease
ExpressionBacterialMutationRNA expression vector for expressing the zinc fin…Available SinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (S939A)
Plasmid#14132DepositorAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano15/pBig
Plasmid#51962PurposeExpression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in DictyosteliumDepositorInsertYellow Cameleon-Nano15
ExpressionBacterialAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1neo-14-3-3-CLuc
Plasmid#107611Purposemeasure LATS activity by Luciferase AssayDepositorInsertpCDNA3.1hygro-BamHI-CLuc1433-NotI
ExpressionMammalianAvailable SinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (T1462A)
Plasmid#14130DepositorAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSG5-ERRbeta2
Plasmid#52186PurposeExpresses human ERRbeta2 splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (ESRRB Human)
ExpressionMammalianMutationwild type sequence; codon optimized to exclude Ec…PromoterSV40Available SinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
tdTomato/pTREX-b
Plasmid#68709PurposeExpresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.DepositorInserttdTomato
UseExpression of tdtomato red fluorescence protein i…Available SinceOct. 8, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2-Flag-TTGZFP-FokI-DD
Plasmid#18755DepositorInsertzinc finger nuclease
ExpressionBacterialMutationRNA expression vector for expressing the zinc fin…Available SinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS2
Plasmid#109289PurposeExpresses high levels of protein in mycobacteriaDepositorTypeEmpty backboneExpressionBacterialMutationpSUM-kan-MCS1-gfp was modified to reorient the Ba…Available SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
mGFAP-Halo
Plasmid#169474Purposethe host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sitesDepositorAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSG424 ELKC
Plasmid#89333PurposeELK1 encoding amino acid 307-428. Cloning sites BamHI and Xba I. Size 365bpDepositorAvailable SinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF
Plasmid#72949PurposeEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteDepositorInsertLevel 0 - ORFs
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)
Plasmid#40341DepositorInsertBlimp1 promoter (mutated) (PRDM1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationTwo AP-1 sites at -1813 and -1647 replaced with B…PromoterBlimp1Available SinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B11)
Plasmid#185859PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRWJB006
Plasmid#160113PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoter35SAvailable SinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB004
Plasmid#160112PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoterUBQ10Available SinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only