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pPB-cT3G-cERP2-PRDM9

Plasmid

#222565

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of PRDM9

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

PRDM9 (PRDM9 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA-TadA*

Plasmid

#125661

Purpose

Expresses the TadA-TadA* dimer in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

TadA-TadA*

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pFF746

Plasmid

#61451

Purpose

aTc-inducible SCRIBE_galK(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(ON)

Use

Synthetic Biology

Promoter

pTetO

Available Since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) rat C/EBP beta (LIP)

Plasmid

#12562

Depositor

Peter Johnson

Insert

CCAAT/Enhancer-binding Protein beta LIP (Cebpb Rat)

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
pFF828

Plasmid

#61456

Purpose

AHL-inducible msd_lacZ(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_lacZ(ON)

Use

Synthetic Biology

Promoter

pLuxR

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pGLx2-SET1

Plasmid

#53256

Purpose

GAL driven LexA-tagged SET1

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Tags

LexA

Expression

Yeast

Promoter

GAL1/10

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pSEVA19_LacI_pLac-sgRNA-1

Plasmid

#231408

Purpose

sgRNA-1 expression from an IPTG-inducible promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

sgRNA-1
LacI

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_AraC_pBAD-sgRNA-1

Plasmid

#231407

Purpose

sgRNA-1 expression from an arabinose-inducible promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

sgRNA-1
AraC

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-1_M13ps

Plasmid

#231409

Purpose

M13 phagemid that expresses sgRNA-1 from a strong constitutive promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-1

Use

Synthetic Biology

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SJZ2-TRP1

Plasmid

#228256

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

TRP1

Expression

Yeast

Promoter

BBC1 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZNF541

Plasmid

#222560

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZNF541

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

ZNF541 (ZNF541 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-NFYB

Plasmid

#222561

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of NFYB

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

NFYB (NFYB Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nMagHigh1-EGFP-CAAX

Plasmid

#67300

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

nMagHigh1-EGFP-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV_BoDV1_G

Plasmid

#207256

Purpose

Mammalian expression of Borna disease virus 1 G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

BoDV1_G

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-Cas9-ScaligD

Plasmid

#125688

Purpose

Gene knockout/in for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spCas9, sgRNA casette, and ScaligD

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah1

Plasmid

#122568

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJKR-H-araC

Plasmid

#62563

Purpose

Arabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-H-acuR

Plasmid

#62567

Purpose

Acrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-L-tetR

Plasmid

#62562

Purpose

Tetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmaxAW

Plasmid

#125647

Purpose

Expresses ABEmaxAW in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A, TadA* V106W)

Expression

Mammalian

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pJKR-H-ttgR

Plasmid

#62565

Purpose

Flavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJKR-L-acuR

Plasmid

#62568

Purpose

Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJKR-L-mphR

Plasmid

#62560

Purpose

Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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BSP611

Plasmid

#122256

Purpose

expression vector for mtagRFP-T under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagRFP-T

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEL856

Plasmid

#214256

Purpose

Expression TadDE-NG in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pFF145

Plasmid

#61446

Purpose

aTc-inducible Beta recombinase

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

Beta recombinase

Use

Synthetic Biology

Promoter

pTetO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pGT-Ah2

Plasmid

#122569

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pFF714

Plasmid

#61449

Purpose

IPTG-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFF706

Plasmid

#61448

Purpose

Light-inducible SCRIBE_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_kanR(ON)

Use

Synthetic Biology

Promoter

pDawn

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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KALRN (KALRNA-c001)

Plasmid

#110256

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

KALRN (KALRN Human)

Tags

His10-TEV

Expression

Bacterial

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5

Plasmid

#61256

Purpose

CRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassette

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

2xTY1-V5

Use

CRISPR

Tags

TY1 and V5

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJKR-L-ttgR

Plasmid

#62566

Purpose

Flavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFF758

Plasmid

#61454

Purpose

IPTG-inducible msd(wt)_dRT

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd(wt)_dRT

Use

Synthetic Biology

Mutation

D197A D198A

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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YIplac211-CHC1-msGFPx3

Plasmid

#105256

Purpose

Plasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Chc1 portion (CHC1 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFF749

Plasmid

#61452

Purpose

IPTG-inducible msd_kanR(ON)_dRT

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)_dRT

Use

Synthetic Biology

Mutation

D197A D198A

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLPC FLAG hRap1 delta Br delta M

Plasmid

#13256

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br delta M (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Mutation

contains hRap1 aa2-44 + 184-399 and the point mut…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pSL6 - Brr2 del247 WT

Plasmid

#90256

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pFF761

Plasmid

#61455

Purpose

aTc-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Promoter

pTetO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVLINK-EF1alpha-HA-5'-IQSEC2

Plasmid

#244256

Purpose

5' AAVLINK plasmid for IQSEC2 expression

Depositor

Zhonghua Lu

Insert

IQSEC2 (IQSEC2 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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We narrowed to 4,360 results for: 256