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Showing: 401 - 440 of 526 results

  8. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid
    #202555
    Purpose
    Synaptotagmin-1 sgRNA2 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202555-plasmid-map-sequence-id-404691

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  9. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid
    #202556
    Purpose
    Synaptotagmin-1 sgRNA3 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202556-plasmid-map-sequence-id-404689

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  10. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid
    #202554
    Purpose
    Synaptotagmin-1 sgRNA1 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202554-plasmid-map-sequence-id-404687

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  15. pResQ shFEN3 3XF-FEN1 wt

    Plasmid
    #17752
    Depositor
    Sheila Stewart
    Insert
    Flap Endonuclease I (FEN1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Triple Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  17. pResQ shFEN3 3XF-FEN1 D181A

    Plasmid
    #17753
    Depositor
    Sheila Stewart
    Insert
    Flap Endonuclease I (FEN1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Triple Flag
    Expression
    Mammalian
    Mutation
    D181A
    Promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  20. KSP_K406

    Plasmid
    #129771
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129771-plasmid-map-sequence-id-263572

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  21. pResQ shFEN3 3XF-FEN1 DeltaC

    Plasmid
    #17754
    Depositor
    Sheila Stewart
    Insert
    Flap Endonuclease I (FEN1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Triple Flag
    Expression
    Mammalian
    Mutation
    Deletion of C-terminal 20 amino acids from FEN1
    Promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  25. pQC V5 HuR.wt IRES Puro

    Plasmid
    #110386
    Purpose
    γ-Retroviral transfer vector for expressing HuR (wild type), IRES-driven Puromycin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110386-plasmid-map-sequence-id-213721

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  29. pQC V5 mKO2 HuR.wt IRES Puro

    Plasmid
    #110387
    Purpose
    γ-Retroviral transfer vector for expressing HuR (wild type), V5 and mKO2 tags, IRES-driven Puromycin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    V5 and mKO2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    110387-plasmid-map-sequence-id-213705

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  32. pELPS-eDHFR-YFP-T2A-Renilla

    Plasmid
    #193253
    Purpose
    Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Depositor
    Mark Sellmyer
    Article
    Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1
    Insert
    The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Use
    Lentiviral
    Tags
    Renilla luciferase (rLuc) and yellow fluorescent …
    Expression
    Mutation
    None
    Promoter
    EF1a
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    193253-plasmid-map-sequence-id-383064

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  39. pQC V5 mKO2 HuR.DelHNS IRES Puro

    Plasmid
    #110389
    Purpose
    γ-Retroviral transfer vector for expressing HuR (delHNS), V5 and mKO2 tags, IRES-driven Puromycin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    V5 and mKO2
    Expression
    Mammalian
    Mutation
    HNS (HuR nuclear-cytoplasmic shuttling sequence) …
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110389-plasmid-map-sequence-id-227209

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Showing: 401 - 440 of 526 results