We narrowed to 1,621 results for: nif
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Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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PLKO-mcherry-luc-puro-Renilla cDNA
Plasmid#29783DepositorInsertRenilla
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
FAB(ER-LD)
Plasmid#214424PurposeDetection of ER-lipid droplet contact siteDepositorInsertFRB-V5
UseTagsCFAST-Halo and RspAN-FRB-V5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CFAST-Halotag-ER
Plasmid#214413PurposeER localization of CFAST fused to Halotag-ERDepositorInsertrat cb5 ERM
UseTagsCFAST-HaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVenus-N-SLC35B2
Plasmid#155038PurposeTransient expression of SLC35B2-VenusDepositorInsertSLC35B2 (SLC35B2 Human)
UseTagsVenusExpressionMammalianMutationSee depositor comments belowPromoterCMVAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-mApple-6xHp
Plasmid#214412PurposeLipid droplet localization of low-affinity NFAST fused to mApple-6xHpDepositorInsertRspA-N
UseTagsmApple-6xHpExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Actb5UTR-renilla
Plasmid#38234DepositorInsertActb 5'UTR / Renilla (Actb Mouse)
UseTagsExpressionMammalianMutationActb 5' UTR fused to renilla ORFPromoterEndogenous ActbAvailable sinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)
Plasmid#184394PurposeTransient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globinDepositorInsertRenilla luciferase
UseTags3X FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXL2
Plasmid#153135PurposeExpression of Flag tagged human FOXL2 in mammalian cellsDepositorInserthuman FOXL2 (FOXL2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis2-Arf1 (17-181)-Q71L
Plasmid#184808PurposeBacterial expression of Arf1 (aa17-181, Q71L mutation)DepositorInsertArf1 (ARF1 Human)
UseTagsHis6ExpressionBacterialMutationaa 17-181 only, Q71LPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXE1
Plasmid#153120PurposeExpression of Flag tagged human FOXE1 in mammalian cellsDepositorInserthuman FOXE1 (FOXE1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…TagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Apr)
Plasmid#110085PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C-SLC35B2
Plasmid#155037PurposeTransient expression of EGFP-SLC35B2DepositorInsertSLC35B2 (SLC35B2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ
Plasmid#192380PurposeTo test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXN3
Plasmid#153139PurposeExpression of Flag tagged human FOXN3 in mammalian cellsDepositorInserthuman FOXN3 (FOXN3 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-G51PEKN
Plasmid#118582PurposeBacterial expression of E.coli DHFR G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationG51 mutated to P51; Glu-Lys-Asn residues inserted…PromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST39-His/Vps27(345-440) - Hse(275-375)
Plasmid#17638DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-Halo-ER
Plasmid#214419PurposeER localization of low-affinity NFAST fused to Halo-ERDepositorInsertRspA-N
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-min5UTR-hRluc-A60
Plasmid#182638PurposeTranscription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequenceDepositorInsertRenilla luciferase,
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUiLMO2W
Plasmid#129480Purpose3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin CAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-iLMO2
Plasmid#129474PurposeMammalian expression vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ
Plasmid#192399PurposeOff state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.DepositorInsertAct2::FRT-Rluc-FRT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ
Plasmid#192398PurposeOn state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.DepositorInsertAct2::FRT-Rluc-FRT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC
Plasmid#192373PurposeTo test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.DepositorInsertAct2::FRT-OCS-TB-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC
Plasmid#192376PurposeTo test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.DepositorInsertAct2::FRT-uORF-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC
Plasmid#192370PurposeTo control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.DepositorInsertAct2::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ
Plasmid#192375PurposeTo test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.DepositorInsertAct2::FRT-35Ster-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ
Plasmid#192374PurposeTo test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.DepositorInsertAct2::FRT-OCStrunc-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ
Plasmid#192385PurposeTo test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.DepositorInsertAct2::B3RT-OCS-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ
Plasmid#192386PurposeTo test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ
Plasmid#192404PurposeOn state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.DepositorInsertB3RT-Act2-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ
Plasmid#192401PurposeOff state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.DepositorInsertAct2::B3RT-Rluc-B3RT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ
Plasmid#192400PurposeOn state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.DepositorInsertAct2::B3RT-Rluc-B3RT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ
Plasmid#192402PurposeOn state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.DepositorInsertFRT-Act2-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ
Plasmid#192405PurposeOff state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.DepositorInsertB3RT-Act2-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ
Plasmid#192403PurposeOff state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.DepositorInsertFRT-Act2-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ
Plasmid#192381PurposeTo test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ
Plasmid#192379PurposeTo test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO
Plasmid#192377PurposeTo test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO
Plasmid#192378PurposeTo test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Myosin7B-sgRNA
Plasmid#154861PurposeLentiviral expression of Cas9 and sgRNA targeting Myosin7BDepositorInsertMYO7B sgRNA (MYO7B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLhE_AW-pHG165
Plasmid#90062PurposeConstitutive expression of a chimeric LacI:CelR transcription repressorDepositorInsertLLhE_AW
UseTagsExpressionBacterialMutationI48V/Q55A/Q60RPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhS-pHG165a
Plasmid#90040PurposeConstitutive expression of a chimeric LacI:GalS transcription repressorDepositorInsertLLhS
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhF-pHG165a
Plasmid#90041PurposeConstitutive expression of a chimeric LacI:FruR transcription repressorDepositorInsertLLhF
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhT-pHG165a
Plasmid#90042PurposeConstitutive expression of a chimeric LacI:TreR transcription repressorDepositorInsertLLhT
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhR-pHG165a
Plasmid#90043PurposeConstitutive expression of a chimeric LacI:RbsR transcription repressorDepositorInsertLLhR
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhA-pHG165a
Plasmid#90044PurposeConstitutive expression of a chimeric LacI:AscG transcription repressorDepositorInsertLLhA
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhE-pHG165
Plasmid#90045PurposeConstitutive expression of a chimeric LacI:CelR transcription repressorDepositorInsertLLhE
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only