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PBS/pU6-HIF1 alpha RNAi plasmid 2

Plasmid

#21104

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

RNAi against hypoxia inducible factor 1alpha

Expression

Mammalian

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCOM4

Plasmid

#49798

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Tags

S-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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-2.5CAT

Plasmid

#25424

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Science. 1992 Apr 24. 256(5056):538-42.

Insert

Myo D Promoter (MYOD1 Human)

Use

Reporter construct

Tags

CAT

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pCAG-hHDAC6

Plasmid

#21099

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

Histone Deacetylase 6 (HDAC6 Human)

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
F3/-2.5CAT

Plasmid

#25425

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Science. 1992 Apr 24. 256(5056):538-42.

Insert

Myo D Promoter + F3 (MYOD1 Human)

Use

Reporter construct

Tags

CAT

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pJExpress401-T4 Lysozyme C54T C97A (WT*)

Plasmid

#224353

Purpose

Expresses T4 lysozyme in E. coli

Depositor

Terry Watt

Article

Toro et al MethodsX. 2015 May 18;2:256-62. doi: 10.

Insert

T4 Lysozyme

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

C45T, C94A

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBES1-LacOx50-Hyg

Plasmid

#210261

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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p177bp-LacOx50-Hyg

Plasmid

#210213

Purpose

Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p177bp-LacOx162-Hyg

Plasmid

#210215

Purpose

Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

162xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-blast

Plasmid

#210221

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-HIF1 alpha

Plasmid

#21101

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-HIF1 alpha K532R

Plasmid

#21102

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

HYPOXIA INDUCIBLE FACTOR 1 ALPHA-K532R MUTANT (HIF1A Human)

Expression

Mammalian

Available Since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only
pBES1-LacOx50-mCh-bla

Plasmid

#210260

Purpose

Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
mCherry

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pbs/pU6-HDAC4 RNAi

Plasmid

#21100

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

RNAi against histone deacetylase4 (HDAC4 Mouse, Human)

Expression

Mammalian

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-HDAC4-IGd2

Plasmid

#22536

Purpose

To be used for the RNAi screening purposes (not for HDAC4 expression); see paper

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

Histone Deacetylase 4 (HDAC4 Human)

Tags

IGd2 = Ires-destabilized GFP

Expression

Mammalian

Available Since

Nov. 9, 2009

Availability

Academic Institutions and Nonprofits only
prDNA spacer-LacOx120-20xMS2-Hyg

Plasmid

#210220

Purpose

Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

120xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSMOX-mNG::LacI-V3

Plasmid

#210224

Purpose

Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

mNeongreen Lac repressor fusion protein

Use

Trypanosoma brucei expression

Promoter

Pol II readthrough transcription after integration

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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prDNA spacer-LacOx50-20xMS2-Hyg

Plasmid

#210219

Purpose

Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-hHDAC4

Plasmid

#21096

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

Histone Deacetylase 4 (HDAC4 Human)

Expression

Mammalian

Mutation

shRNA target sequence was mutated (mutated nucleo…

Promoter

CAG

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-hHIF1 alpha-IGd2

Plasmid

#22535

Purpose

To be used for the RNAi screening purposes (not for HDAC4 expression); see paper

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

hypoxia inducible factor 1 alpha (HIF1A Human)

Expression

Mammalian

Available Since

Nov. 9, 2009

Availability

Academic Institutions and Nonprofits only