We narrowed to 10,893 results for: ENA;

1. pAGM9121_Mro-UPO SP

   Plasmid

   #153499

   Purpose

   Marasmius rotula Unspecific Peroxygenase Signal Peptide Donor Plasmid

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

   Insert

   Unspecific peroxygenase SP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcF-1/3/2

   Plasmid

   #131026

   Purpose

   Three-spacer array 2 for FnCas12a.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Insert

   3-spacer array for FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcF-1/2/3

   Plasmid

   #131027

   Purpose

   Three-spacer array 1 for FnCas12a.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Insert

   3-spacer array for FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcF-2

   Plasmid

   #131025

   Purpose

   Single spacer array with spacer #2 for FnCas12a.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Insert

   single spacer array for FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcF-3

   Plasmid

   #131024

   Purpose

   Single spacer array with spacer #3 for FnCas12a.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Insert

   single spacer array for FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFncpf1_7_spacer_array

   Plasmid

   #131032

   Purpose

   Seven-spacer array for FnCas12a.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Insert

   7-spacer array for FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcF-3/2/1

   Plasmid

   #131031

   Purpose

   Three-spacer array 6 for FnCas12a.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Insert

   3-spacer array for FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcF-2/3/1

   Plasmid

   #131030

   Purpose

   Three-spacer array 5 for FnCas12a.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Insert

   3-spacer array for FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcF-3/1/2

   Plasmid

   #131029

   Purpose

   Three-spacer array 4 for FnCas12a.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Insert

   3-spacer array for FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcF-2/1/3

    Plasmid

    #131028

    Purpose

    Three-spacer array 3 for FnCas12a.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Insert

    3-spacer array for FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPD1R

    Plasmid

    #86969

    Purpose

    Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH

    Promoter

    T7 promoter

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pfMDH_Triple

    Plasmid

    #67733

    Purpose

    Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed histidine 303 to alanine, arginine 373 to…

    Promoter

    T7

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pFnCpf1GG

    Plasmid

    #131010

    Purpose

    Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of FnCas12a
    GFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE60 Q58L wgMDH

    Plasmid

    #204246

    Purpose

    Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Q58L

    Promoter

    T5

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCXMG

    Plasmid

    #204409

    Purpose

    Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

    Depositor

    David Ackerley

    Article

    Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AviTag-SpyTag003-MBP

    Plasmid

    #184227

    Purpose

    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    AviTag-SpyTag003-MBP
    

    Use

    Tags

    AviTag, His6, SpyTag003, and Thrombin cleavage si…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE60 R130A wgMDH

    Plasmid

    #204266

    Purpose

    Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130A

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQE60 R124K wgMDH

    Plasmid

    #204258

    Purpose

    Bacterial expression of R124K wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124K

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQE60 R124Q wgMDH

    Plasmid

    #204259

    Purpose

    Bacterial expression of R124Q wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124Q

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQE60 R124A wgMDH

    Plasmid

    #204257

    Purpose

    Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124A

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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