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Showing: 201 - 220 of 2911 results

  1. pPlacPbuCas13b-gRNA-T-deGFP

    Plasmid
    #184840
    Purpose
    Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and lac promoter
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    184840-plasmid-map-sequence-id-373961

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  2. APPL1-N308D-GFP

    Plasmid
    #102783
    Purpose
    Point mutation in APPL1 that diminishes its interaction with Rab5
    Depositor
    Donna Webb
    Insert
    APPL1 (APPL1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    changed asparagine 308 to aspartate; *(see below)
    Promoter
    CMV
    Available since
    Jan. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    102783-plasmid-map-sequence-id-198005

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  9. mApple-proα2(I)-G610C

    Plasmid
    #119841
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mApple between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119841-plasmid-map-sequence-id-233868

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  10. APPL1-N308D-mCherry

    Plasmid
    #102784
    Purpose
    Point mutation in APPL1 that diminishes its interaction with Rab5
    Depositor
    Donna Webb
    Insert
    APPL1 (APPL1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed asparagine 308 to aspartate; *(see below)
    Promoter
    CMV
    Available since
    Jan. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    102784-plasmid-map-sequence-id-197993

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  11. mApple-Talin-ABM-10

    Plasmid
    #54950
    Purpose
    Localization: Talin Actin Binding Domain, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Talin ABM
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Actin Binding Module; I/LWEQ = 197 amino acids fr…
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 201 - 220 of 2911 results