We narrowed to 225 results for: YPet
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Plasmid#205568PurposeRasAR negative-control mutant plasmid. Targeted to cytosol.DepositorInsertRasAR(R89L)-NES (RAF1 Human, Synthetic)
TagsNuclear export signal (NES)ExpressionMammalianMutationArg 89 mutated to Leu in Raf1 RBD region.PromoterCMVAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE212.pa
Plasmid#134930PurposeGEF-H1 biosensor that does not have GEF activity.DepositorInsertGEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
TagsNone.ExpressionBacterial and MammalianPromoterCMV and T7Available SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE191.pa
Plasmid#134931PurposeGEF-H1 biosensor that has GEF activity.DepositorInsertGEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
TagsNone.ExpressionBacterial and MammalianPromoterCMV and T7Available SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
beta catenin binding mutant of alpha catenin sensor
Plasmid#71711PurposeThis form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctionsDepositorInsertalpha-catenin Δβ-cat mutant FRET Sensor
TagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 48- 163PromoterCMVAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
VBS mutant of alpha catenin sensor
Plasmid#71710PurposeThis form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.DepositorInsertalpha-catenin ΔVBS FRET Sensor
TagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 316-405 in α -catenin…PromoterCMVAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr-6080NES(Epac-sensor)
Plasmid#162487PurposehyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertEpac-sensor (RAPGEF3 Human, Synthetic)
TagsYPet and Turquoise2GLExpressionMammalianPromoterCAGAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Golgi-RasAR
Plasmid#205570PurposeGolgi membrane-targeted RasAR.DepositorTagsGolgin B1 (Giantin) residues 3131-3259ExpressionMammalianPromoterCMVAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b-M3C
Plasmid#107087PurposeM. sedula PCNA3 fused at the N-terminus of CyPetDepositorInsertM. sedula PCNA3 fused to CyPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-S3C
Plasmid#107090PurposeS. solfataricus PCNA3 fused at the N-terminus of CyPetDepositorInsertS. solfataricus PCNA3 fused to CyPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-S1C
Plasmid#107088PurposeS. solfataricus PCNA1 fused at the N-terminus of CyPetDepositorInsertS. solfataricus PCNA1 fused to CyPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-M2C
Plasmid#107086PurposeM. sedula PCNA2 fused at the N-terminus of CyPetDepositorInsertM. sedula PCNA2 fused to CyPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-M1C
Plasmid#107085PurposeM. sedula PCNA1 fused at the N-terminus of CyPetDepositorInsertM. sedula PCNA1 fused to CyPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
JH05
Plasmid#73516PurposemYPet_PH pCFJ150 - contain C. elegans codon optimized mYPetDepositorInsertpmex-5::mPYet::PH::tbb-2
TagsmYPetPromotermex-5Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
H3K9me3 biosensor
Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
ExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
ExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
ExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR T757
Plasmid#140828PurposeAIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cellsDepositorInsertAIMTOR T757 (ULK1 Human)
TagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…Available SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-CFP
Plasmid#90103PurposeCFP control expression plasmidDepositorInsertCFP
UseBroad-host rangeExpressionBacterialPromoterPtacAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
LT2_15_UPRpro_X2site
Plasmid#194427PurposePlasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein ResponseDepositorInsertUPRpro
UseUsed as donor dna in genome integration after lin…ExpressionYeastMutationNAAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT2_33_PyruPro_X2site
Plasmid#194429PurposePlasmid for genome integration in X2 site expressing PyruPro (Pyruvate consumption Probe)DepositorInsertPyruPro
UseUsed as donor dna in genome integration after lin…ExpressionYeastMutationNAAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only