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phCMV-GALV-MTR

Plasmid

#163612

Purpose

Retro and Lentiviral transduction of primary human germinal center B cells

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Insert

GaLV MTR envelope

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2-GAL3

Plasmid

#149518

Purpose

Express APEX2-Galectin-3 in mammalian cells

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS3 (LGALS3 Human)

Use

Tags

APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16-HSF1 WT

Plasmid

#71726

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 VP16 AD (HSF1 Budding Yeast, Human, Herpes Simplex Virus protein VP16)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGAL-SKI1-NAT

Plasmid

#160382

Purpose

Overxpression of SKI1/XRN1 in yeast

Depositor

John McCusker

Insert

SKI1

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-GAL4-DBD

Plasmid

#138394

Purpose

RMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4-DBD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pU6Rosa-CAG-Gal4-Rad18UBD

Plasmid

#119012

Purpose

Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Gal4 (GAL4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAG425 GAL Pong TPase L418A, L420A

Plasmid

#145795

Purpose

mPing Yeast Transposition Assay

Depositor

Susan Wessler, Charles Nathan Hancock

Article

Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

Insert

Pong TPase L418A, L420A

Use

Tags

Expression

Yeast

Mutation

L418A, L420A

Promoter

Available since

Jan. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHCA/GAL4(848).ER

Plasmid

#108215

Purpose

Yeast expression vector for fusion of Gal4 AA 1-848 to hormone binding domain of estrogen receptor a

Depositor

Didier Picard

Insert

Gal4-ERalpha HBD

Use

Tags

Expression

Yeast

Mutation

ERalpha HBD has G400V mutation

Promoter

ADH0

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBMPGAL4LWL

Plasmid

#26270

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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GAL-Aur1-2GFP-Hygromycin

Plasmid

#184775

Purpose

Yeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

proGAL-Aur1 (AUR1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CAG-mRuby3-Gal8-P2A-Zeo

Plasmid

#150815

Purpose

Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:

Insert

mRuby3-Gal8-P2A-Zeo (LGALS8 Synthetic, Human)

Use

Tags

Gal8 is fused to the c-terminus of mRuby3

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCEFL GAL4dbd-KLF4 159-329

Plasmid

#140148

Purpose

GAL4-DNA binding domain fusion protein with amino acids 159 to 329 of human KLF4 (repressor domain)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 aa 159-329 (KLF4 Human)

Use

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 1 to 158 and 330 to 479

Promoter

EF1a

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)

Plasmid

#203909

Purpose

Encodes wild type Gal4-VP64 transcription factor in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4-VP64-IRES-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVd1-NLS-GAL4-iLight2-VP16

Plasmid

#219550

Purpose

NLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16

Use

AAV

Tags

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-PGAL-GFP-Cps1

Plasmid

#207030

Purpose

Expression of GFP-Cps1 under GAL promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Cps1

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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GALTA-c001

Plasmid

#79645

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GALT (GALT Human)

Use

Tags

6x His and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pHES553 [prGal1-RAS2(KAN) pnh604]

Plasmid

#103846

Purpose

Yeast Expression Plasmid

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

pGal1-Ras2(KAN)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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M15 pCS-twhh-nBeta gal

Plasmid

#17152

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

twhh promoter (shhb Zebrafish)

Use

Tags

nuclear beta-gal

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pMK419 (GAL-OsTIR1(F74G))

Plasmid

#140656

Purpose

GAL-OsTIR1(F74G)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

GAL-OsTIR1(F74G)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK425 (GAL-OsTIR1(F74A))

Plasmid

#140657

Purpose

GAL-OsTIR1(F74A)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

GAL-OsTIR1(F74A)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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