Addgene

We narrowed to 1,089 results for: ncl

Showing: 201 - 220 of 1089 results

Results per page:

Sort results by:

pJMP1273

Plasmid

#119265

Purpose

E. coli attTn7 site with B. subtilis flanking sequence cloned into pACYC

Depositor

Jason Peters, Oren Rosenberg, Carol Gross

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

E. coli attTn7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP1263

Plasmid

#119264

Purpose

B. subtilis attTn7 site and flanking sequence cloned into pACYC

Depositor

Jason Peters, Oren Rosenberg, Carol Gross

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

B. subtilis attTn7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP1106

Plasmid

#119249

Purpose

construction intermediate

Depositor

Jason Peters, Oren Rosenberg, Carol Gross

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDECKO_UCA1

Plasmid

#72628

Purpose

Expresses two gRNAs targeting the UCA1 promoter

Depositor

Rory Johnson, Roderic Guigo

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward UCA1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-FLAG-RHEB-IRES-Puro

Plasmid

#120576

Purpose

Expresses Flag-RHEB fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP_EHMT1V09

Plasmid

#228813

Purpose

Expression of mouse EHMT1 with deletion of aa 1-31

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 1-31

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNDGG010

Plasmid

#231315

Purpose

Vector part for BEVA; BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNDGG009

Plasmid

#231314

Purpose

Vector part for BEVA; BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG NC

Plasmid

#214675

Purpose

Negative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tags

Depositor

Eugene Yeo

Article

Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

FLAG

Use

Tags

MCP and V5

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOME0135

Plasmid

#178397

Purpose

KSHV Kaposincluster-RevCompl-Syn

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

Kaposincluster-RevCompl-SYN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOME234M

Plasmid

#178416

Purpose

Reverse compl sequence of KSHV Kaposincluster-RevCompl-Syn for pcDNA3.1-

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

Reverse complement Kaposincluster-RevCompl-SYN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOME0235M

Plasmid

#178417

Purpose

KSHV Reverse compl Sequence Kaposincluster_RevCompl-KapATGdel_Syn

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

ReverseComplement-Kaposincluster_RevCompl_KapATGdel_Syn

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8_TaNAM-A1

Plasmid

#124574

Purpose

TaNAM-A1 CDS in the Gateway-compatible pCR8 vector.

Depositor

Cristobal Uauy

Article

Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

Insert

TaNAM-A1

Use

Topo gateway cloning vector

Tags

Expression

Mutation

Promoter

None

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-FHH-MAPKAP1RBD

Plasmid

#120710

Purpose

Expresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 279-353

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-Ubiquitin-KO

Plasmid

#17603

Purpose

Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

KO: all lysines mutated to arginines

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-OSBP-PH

Plasmid

#49571

Purpose

expresses the plekstrin homology domain of OSBP in mammalian cells

Depositor

Marci Scidmore

Article

Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

Insert

OSBP-PH (AA 91-179) (OSBP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p6350 MSCV-CMV-CMV-Flag-HA-ATAD5

Plasmid

#31356

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

ATAD5 (ATAD5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
p6352 MSCV-CMV-CMV-Flag-HA-JMJD6

Plasmid

#31358

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

JMJD6 (JMJD6 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pTargeting_Mettl3C_FKBP-V

Plasmid

#165421

Purpose

Template for knockin FBKP-V into Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

Mettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)

Use

Mouse Targeting

Tags

FKBP-V

Expression

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP1339

Plasmid

#119271

Purpose

for cloning new sgRNAs

Depositor

Jason Peters, Oren Rosenberg, Carol Gross

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge