We narrowed to 325 results for: pet-28
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Plasmid#65613Purposeexpress soluble His-tagged zebrafish CSF3b/Gcsfb in E. coliDepositorAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by
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dFP-mini
Plasmid#120543PurposeExpresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.DepositorInsertde novo fluorescence-activating protein mini (dFP-mini)
Tags6xHis - TEV cleavageExpressionBacterialPromoterT7Available sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
His6-EGFP-PH-PLCD1
Plasmid#183675PurposePtdIns(4,5)P2 biosensor for purification from bacteriaDepositorInsertPLCD1 (PLCD1 Human)
TagsHis6-EGFPExpressionBacterialMutationAmino acids 11-140PromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A32
Plasmid#191241PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (HLA-A Human, Staphylococcus aureus)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A31
Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (HLA-A Human, Staphylococcus aureus)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-CP8B1
Plasmid#191240PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (CYP8B1 Human, Staphylococcus aureus)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis-Laforin
Plasmid#157989PurposeExpresses Laforin in E.coli cellDepositorPublicationAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAY02
Plasmid#13043DepositorInsertMutS
TagsHis6ExpressionBacterialMutationT193TT to G193CT Phe to Ala mutationAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP574
Plasmid#222953PurposeExpresses R120A LoaPDepositorInsertantiterminator protein LoaP
Tags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 120 to AlaninePromoterT7Available sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
TagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ALIX(P801G)
Plasmid#180025PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)
TagsGB1-His6-TEVExpressionBacterialMutationP801GAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
dFP
Plasmid#120542PurposeExpresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.DepositorInsertde novo fluorescence-activating protein (dFP)
Tags6xHis - TEV cleavageExpressionBacterialPromoterT7Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT-36
Plasmid#16252DepositorAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
His6-SMT3-SopB
Plasmid#183676PurposeRecombinant Salmonella Typhimurium SopBDepositorInsertsopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
TagsHis6-S. cerevisiae SMT3(2-101)ExpressionBacterialPromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
His6-SMT3-SopB(C460S)
Plasmid#183677PurposeRecombinant Salmonella Typhimurium SopB (catalytically inactive)DepositorInsertsopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
TagsHis6-S. cerevisiae SMT3(2-101)ExpressionBacterialMutationC460SPromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(800-868)-strep
Plasmid#180023PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.DepositorInsertGB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)
TagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
HSA3_His
Plasmid#202765Purposehuman serum albumin design HSA3, in pET29b with C-terminal 6His-tagDepositorInsertHSA3
TagsHis tagExpressionBacterialPromoterT7Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSKB2-TRexR90D
Plasmid#31350DepositorInsertThermus aquaticus Redox Sensing Repressor (R90D)
TagsHisExpressionBacterialMutationchanged Arginine 90 to Aspartic AcidAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
HSA1_His
Plasmid#202763Purposehuman serum albumin design HSA1, in pET29b with C-terminal 6His-tagDepositorInsertHSA1
TagsHis tagExpressionBacterialPromoterT7Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP578
Plasmid#222957PurposeExpresses R144S LoaPDepositorInsertantiterminator protein LoaP
Tags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 144 to SerinePromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only