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pDD56

Plasmid

#119883

Purpose

Arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_EYFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDD57

Plasmid

#119882

Purpose

Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pConstitutive_GFP+

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDD143

Plasmid

#119880

Purpose

Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_dCas9sth1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pVRa34_1384

Plasmid

#49693

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF34_1384

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa38_1322

Plasmid

#49698

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF38_1322

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa27_4265

Plasmid

#49682

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF27_4265

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa17_1458

Plasmid

#49671

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF17_1458

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa14_1324

Plasmid

#49665

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF14_1324

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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tph-1(short)::rpl-22-HA

Plasmid

#122203

Purpose

C. elegans tagged ribosome expression plasmid

Depositor

Steven Flavell

Article

Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

Insert

tph-1(short)::rpl-22-HA

Use

Tags

Expression

Mutation

Promoter

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-VN155(I153L)-TRP1

Plasmid

#53193

Purpose

chromosomal tagging with Venus

Depositor

John Pringle

Article

Onishi et al J Cell Biol. 2013 Jul 22;202(2):311-29.

Insert

Venus

Use

Yeast genomic targeting

Tags

TRP1

Expression

Mutation

aa 1–155, I to L mutation at aa 153

Promoter

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Use

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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UAS-fog

Plasmid

#41178

Depositor

Norbert Perrimon

Article

Hacker et al Genes Dev. 1998 Jan 15;12(2):274-84.

Insert

fog (fog Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-DAAO

Plasmid

#60344

Purpose

Yeast D-Amino acid oxidase from Rhodotorula gracilis, optimized for mammalian expression.

Depositor

Vsevolod Belousov

Article

Matlashov et al Antioxid Redox Signal. 2014 Mar 1;20(7):

Insert

DAAO

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLOX-Ttag-iresTK

Plasmid

#12246

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

SV40 Large T, HSV1-TK (SV40gp6 Simian virus)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pK18-hACE2

Plasmid

#149449

Purpose

Expresses human ACE2 driven by the K18 promoter

Depositor

Paul McCray

Article

McCray PB et al J Virol. 2007 Jan;81(2):813-21. doi: 10.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

K18

Available since

June 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-hChR2(H134R)-EYFP

Plasmid

#114367

Purpose

expresses channelrhodopsin 2 fused with EYFP

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

hChR2(H134R)

Use

Tags

EYFP

Expression

Mammalian

Mutation

the Histidine at amino acid position 134 was chan…

Promoter

pCAG

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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VV201: ChR2(C128S)-eGFP in fubi

Plasmid

#58510

Purpose

Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

ChR2(C128S)-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Cysteine 128 to Serine

Promoter

Ubiquitin

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP

Plasmid

#47905

Purpose

An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

hChR2(H134R)

Use

AAV

Tags

iRFP

Expression

Mammalian

Mutation

H134R

Promoter

CaMKIIa

Available since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE

Plasmid

#20294

Purpose

Lentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

Depositor

Karl Deisseroth

Article

Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

changed Cysteine 128 to Serine

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

Plasmid

#20293

Purpose

Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

Depositor

Karl Deisseroth

Article

Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

changed Cysteine 128 to Alanine

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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