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We narrowed to 211 results for: tet rtTA

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Showing: 201 - 211 of 211 results

  1. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…
    Promoter
    CAG, EF1a, and TRE3G
    Available Since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    198397-plasmid-map-sequence-id-389535

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  6. pUt-mPF-GFP

    Plasmid
    #199222
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    rtTA-Advanced::P2A::EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    tight TRE promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199222-plasmid-map-sequence-id-396540

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  7. pHPHS232

    Plasmid
    #228356
    Purpose
    Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d
    Inserts
    mTagBFP2
    Reverse tetracycline transactivator 3
    neo
    Tags
    Blasticidin S deaminase and iCasp9
    Expression
    Mammalian
    Promoter
    TRE3GV and gene trap
    Available Since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228356-plasmid-map-sequence-id-455149

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  8. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid
    #199221
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    tight TRE promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199221-plasmid-map-sequence-id-396541

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  10. FUT8-Dox

    Plasmid
    #124631
    Purpose
    Doxycycline-inducible FUT8 expression in mammalian cells
    Depositor
    Ron Weiss
    Article
    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038
    Inserts
    Alpha-(1,6)-fucosyltransferase
    Tetracycline repressor A3 mutant
    TagFP635
    Expression
    Mammalian
    Promoter
    TRE-tight and hEF1a
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124631-plasmid-map-sequence-id-248079

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  11. B4GALT1-Dox

    Plasmid
    #124633
    Purpose
    Doxycycline-inducible B4GALT1 expression in mammalian cells
    Depositor
    Ron Weiss
    Article
    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038
    Inserts
    Beta-(1,4)-Galactosyltransferase 1
    Tetracycline repressor A3 mutant
    TagFP635
    Expression
    Mammalian
    Promoter
    TRE-tight and hEF1a
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124633-plasmid-map-sequence-id-248076

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Showing: 201 - 211 of 211 results