We narrowed to 13,644 results for: NTS

1. pGEX4T-E2-25K

   Plasmid

   #63572

   Purpose

   Expression of human Ubiquitin conjugating enzyme E2-25K

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBE2-25K (UBA7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Isoform 1 (200 resiudes)

   Promoter

   tac

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

2. Lenti-EF1alpha-dCas9-p300_Blast

   Plasmid

   #192653

   Purpose

   3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. S100A6

   Plasmid

   #196217

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses S100A6.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   S100A6 (S100A6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMU-ERr

   Plasmid

   #61170

   Purpose

   Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   AtWAK2-mCherry-HDEL
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   April 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSF279

   Plasmid

   #167674

   Purpose

   Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRU319

   Plasmid

   #167686

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMOD_A1111

   Plasmid

   #91032

   Purpose

   Module A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TaCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   OsAct1

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZac2.1 gfaABC1D-cyto-GCaMP6f

   Plasmid

   #52925

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Baljit Khakh

   Article

   Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

   Has Service

   AAV5

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCI-neo FlucSM EGFP

   Plasmid

   #90171

   Purpose

   EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

   Depositor

   Franz-Ulrich Hartl

   Article

   Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

   Insert

   FLuc EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R188Q in FLuc

   Promoter

   Available since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-myc-CUL5

    Plasmid

    #19895

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 5 (CUL5 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFG2

    Plasmid

    #87250

    Purpose

    gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    Nat
    

    Use

    Tags

    parS segments

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SaKKH-ABEmax

    Plasmid

    #119815

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSaCas9 (KKH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Adeno Cas9

    Plasmid

    #64072

    Purpose

    Adenoviral vector for the expression of the Cas9

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    U6 and CBh

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPEG

    Plasmid

    #162466

    Purpose

    Entry vector for gRNA insertion in plant prime editing expression vector

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Type

    Empty backbone

    Use

    Entry vector for grna

    Tags

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ141B2.0

    Plasmid

    #99897

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRDA_078

    Plasmid

    #158582

    Purpose

    Lentiviral expression of BE4max and customizable gRNA

    Depositor

    David Root, John Doench

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 D10A

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAB1

    Plasmid

    #62547

    Purpose

    broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

    Depositor

    Michael Franklin

    Article

    Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

    Insert

    eGFP
    

    Use

    Broad host range plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLAI-Env

    Plasmid

    #133996

    Purpose

    Env gene from HIV-1 LAI in pcDNA3.1 expression vector

    Depositor

    Himanshu Garg

    Article

    Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

    Insert

    Envelope Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAT15489_dABE8e

    Plasmid

    #174127

    Purpose

    plasmid expressing ABE8e with dCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    dABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACS141

    Plasmid

    #204597

    Purpose

    Histone H3.3-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    human histone H3.3 (H3-3A Human)
    

    Use

    Tags

    human histone H3.3 - V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGTQL1221YC

    Plasmid

    #61705

    Purpose

    Gateway compatible BiFC vector (pEarleyGate202-YC)

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Bifc assay

    Tags

    YFP-C and FLAG

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p2T-CMV-ABEmax-BlastR

    Plasmid

    #152989

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    ABE7.10-nSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pComb3XLambda

    Plasmid

    #63892

    Purpose

    Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Insert

    human antibody lambda light chain (IGLL5 Human)
    

    Use

    Phage display

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. UBQ10:sXVE:(MCS)-S11-DI-GFP1–9

    Plasmid

    #108260

    Purpose

    Binary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    UBQ10:sXVE:

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHR iRFP-ZtSH2

    Plasmid

    #188627

    Purpose

    Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    ZtSH2
    

    Use

    Lentiviral

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-RLuc8.6-535

    Plasmid

    #87125

    Purpose

    Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

    Depositor

    Sanjiv Sam Gambhir

    Article

    Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

    Insert

    Renilla Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEHlyA5

    Plasmid

    #168298

    Purpose

    Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

    Depositor

    Luis Ángel Fernández

    Article

    Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

    Insert

    N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
    

    Use

    Tags

    E-tag, HA-tag, His-tag, Prescission site, and VHH…

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CamKIIa-moxBFP-WPRE

    Plasmid

    #194976

    Purpose

    AAV vector to drive expression of moxBFP under the control of CamKIIalpha promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    moxBFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRDA_256

    Plasmid

    #158581

    Purpose

    Lentiviral expression of BE3.9max and customizable gRNA

    Depositor

    David Root, John Doench

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 D10A

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMSR0

    Plasmid

    #161984

    Purpose

    Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

    Depositor

    Olga Soutourina

    Article

    Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Clomeleon

    Plasmid

    #203723

    Purpose

    A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

    Depositor

    George Augustine

    Article

    Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

    Insert

    cyan fluorescent protein linked yellow florescent protein
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Cyan Fluorescent Protein, Topaz Fluorescent Prote…

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PKD1-F (pCI-PKD1-Flag)

    Plasmid

    #21369

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGR

    Plasmid

    #46002

    Purpose

    Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GFP-itis pSel

    Plasmid

    #195344

    Purpose

    Selection plasmid expressing non-fluorescent EGFP A111V

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    A111V

    Promoter

    J23119

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTW2065

    Plasmid

    #236574

    Purpose

    ISYmu1 PDS3 gRNA2 TRV2 Architecture_A

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    ISYmu1 TnpB
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFG4

    Plasmid

    #87251

    Purpose

    gene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    Hyg
    

    Use

    Tags

    parS segments

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FusionRed-pBAD

    Plasmid

    #54677

    Purpose

    Localization: Protein Expression Vector , Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Type

    Empty backbone

    Use

    Tags

    6xHis and FusionRed

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

38. pCD-Ugi22

    Plasmid

    #207004

    Purpose

    pGGC000 Greengate entry vector containing Utricularia gibba insulator 22 (Ugi22) sequence (ID2547)

    Depositor

    Wayne Parrott

    Article

    Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.

    Insert

    Utricularia gibba insulator 22
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pCD-Ugi21

    Plasmid

    #207005

    Purpose

    pGGC000 Greengate entry vector containing Utricularia gibba insulator 21 (Ugi21) sequence (ID9063)

    Depositor

    Wayne Parrott

    Article

    Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.

    Insert

    Utricularia gibba insulator 21
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. p1D4-hrGFP II

    Plasmid

    #71317

    Purpose

    expression vector with 1D4 epitope added as a C-terminal tag, allowing for in vitro expression of any visual pigment or GPCR of interest.

    Depositor

    Belinda Chang

    Article

    Morrow et al Plasmid. 2010 Nov;64(3):162-9. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    1D4 epitope (TETSQVAPA) and IRES-hrGFPII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only