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Showing: 5451 - 5500 of 8645 results

  1. pZNF211-donor

    Plasmid
    #112382
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF211 with GFP
    Depositor
    Kevin White
    Insert
    ZNF211 homology arms flanking EGFP-IRES-Neo cassette (ZNF211 Human)
    Use
    CRISPR
    Mutation
    hg38:19:57641976:T>C, hg38:19:57641979:A>T,…
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112382-plasmid-map-sequence-id-229835

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  2. pZNF766-donor

    Plasmid
    #112352
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF766 with GFP
    Depositor
    Kevin White
    Insert
    ZNF766 homology arms flanking EGFP-IRES-Neo cassette (ZNF766 Human)
    Use
    CRISPR
    Mutation
    hg38:19:52290357:G>T, hg38:19:52290373:A>G,…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112352-plasmid-map-sequence-id-229667

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  3. pZNF84-donor

    Plasmid
    #112330
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF84 with GFP
    Depositor
    Kevin White
    Insert
    ZNF84 homology arms flanking EGFP-IRES-Neo cassette (ZNF84 Human)
    Use
    CRISPR
    Mutation
    rs623100, hg38:12:133058872:T>C, hg38:12:13305…
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112330-plasmid-map-sequence-id-228558

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  4. pTBPL1-donor

    Plasmid
    #112316
    Purpose
    CRISPR donor plasmid to tag human transcription factor TBPL1 with GFP
    Depositor
    Kevin White
    Insert
    TBPL1 homology arms flanking EGFP-IRES-Neo cassette (TBPL1 Human)
    Use
    CRISPR
    Mutation
    rs35776966, hg38:6:133987646:GTGTAT>-
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112316-plasmid-map-sequence-id-228480

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  5. pSMAD3-donor

    Plasmid
    #112311
    Purpose
    CRISPR donor plasmid to tag human transcription factor SMAD3 with GFP
    Depositor
    Kevin White
    Insert
    SMAD3 homology arms flanking EGFP-IRES-Neo cassette (SMAD3 Human)
    Use
    CRISPR
    Mutation
    hg38:15:67189762:C>T, rs11629568, rs8025774
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112311-plasmid-map-sequence-id-228463

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  23. FN 221

    Plasmid
    #50498
    Depositor
    Kenneth Yamada
    Insert
    fibronectin (FN1 Human)
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    repeats 6-10, R1524K, D1526E, C1232A
    Promoter
    T7
    Available Since
    March 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. pBH504

    Plasmid
    #215782
    Purpose
    NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC gene
    Depositor
    Corey Wilson
    Article
    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:
    Inserts
    LacI(YQR)
    sgRNA-ribozyme cassette
    dCas9
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Bt1311, P(O1), and P1
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215782-plasmid-map-sequence-id-430549

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  32. pAd5-B6/7 ∆E3-GFP

    Plasmid
    #179202
    Purpose
    A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6∆E3-GFP and pAd5-B7.
    Depositor
    Fred Bunz
    Article
    Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do
    Insert
    Modified block 6/7, with deletion in E3 and insertion of GFP cassette
    Use
    Adenoviral
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    179202-plasmid-map-sequence-id-351756

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  34. pSUC01

    Plasmid
    #177204
    Purpose
    chromogenic reporter for gene knockout in Streptomyces
    Depositor
    Wenjun Guan
    Article
    Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1
    Insert
    SaindC
    Use
    Synthetic Biology
    Tags
    NONE
    Expression
    Bacterial
    Mutation
    NONE
    Promoter
    TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    177204-plasmid-map-sequence-id-382362

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  41. pSF18

    Plasmid
    #222593
    Purpose
    BB2 ZWF1 tet-on overexpression cassette
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    ZWF1
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    tet-on
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222593-plasmid-map-sequence-id-443809

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  43. pZA16mflon

    Plasmid
    #75439
    Purpose
    Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    mf-lon
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75439-plasmid-map-sequence-id-147407

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  44. psLIB CMV

    Plasmid
    #229953
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229953-plasmid-map-sequence-id-461575

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  48. pST50Trc1-HISNDHFR

    Plasmid
    #63940
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63940-plasmid-map-sequence-id-177346

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  49. pST50Trc3-HISNDHFR

    Plasmid
    #63976
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63976-plasmid-map-sequence-id-177382

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  50. pST50Trc4-HISNDHFR

    Plasmid
    #63994
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63994-plasmid-map-sequence-id-177400

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Showing: 5451 - 5500 of 8645 results