We narrowed to 10,744 results for: AGA

1. AcGFP-MLH1

   Plasmid

   #215120

   Purpose

   Expresses AcGFP-MLH1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   MLH1 (MLH1 Human)
   

   Use

   Retroviral

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMG059 MBP-CREB-His

   Plasmid

   #154076

   Purpose

   Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   CREB1 (CREB1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-MAVS(IRSp53 pLxIS)

   Plasmid

   #221315

   Purpose

   Expression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   MAVS chimera with IRSp53 pLxIS domain (Mavs Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

   Plasmid

   #176459

   Purpose

   Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-PNKP

   Plasmid

   #215137

   Purpose

   Expresses mCherry-PNKP in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PNKP (PNKP Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CCND2 targeting gRNA

   Plasmid

   #215318

   Purpose

   Expresses gRNA targeting CCND2 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CCND1 targeting gRNA

   Plasmid

   #215153

   Purpose

   Expresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. FokI(inactive)-mCherry-LacI-NLS

   Plasmid

   #215100

   Purpose

   Expresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   FokI(inactive)-mCherry-LacI-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   FokI(inactive) mutant D69A.

   Promoter

   CMV

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CDK2 gRNA (BRDN0001148866)

   Plasmid

   #77191

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK2 (CDK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PLL5.0-Anillin ShRNA-GFP-AnillinFL

    Plasmid

    #187272

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6, CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti_CRISPRi_sgAR1

    Plasmid

    #167000

    Purpose

    Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    AR Promoter sgRNA 1 (AR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-AR-R405A

    Plasmid

    #126052

    Purpose

    Expresses human androgen receptor (R405A) in mammalian cells

    Depositor

    Elizabeth Wilson

    Article

    Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

    Insert

    AR (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R405A

    Promoter

    CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLCHKO_intergenic-intergenic_1

    Plasmid

    #155077

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSico_U6-PLOD2 sgRNA4

    Plasmid

    #136459

    Purpose

    Expression of gRNA against human PLOD2

    Depositor

    Antonio Amelio

    Article

    Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

    Insert

    gRNA against human PLOD2 (PLOD2 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAMK2D gRNA (BRDN0001146878)

    Plasmid

    #77186

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CAMK2D

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMG035 MBP-Axin∆BCD-His

    Plasmid

    #154053

    Purpose

    Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pATP416-­pluxO5-­ptetO7-­crtYBI

    Plasmid

    #165975

    Purpose

    Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Inserts

    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    C1281A, G1698A and C66A, C1359T

    Promoter

    Synthetic promoter (pluxO5), Synthetic promoter (…

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-AR-R405S

    Plasmid

    #126051

    Purpose

    Expresses human androgen receptor (R405S) in mammalian cells

    Depositor

    Elizabeth Wilson

    Article

    Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

    Insert

    AR (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R405S

    Promoter

    CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTwist-FLAG-SRSF3_Truncated_Codon.Optimized

    Plasmid

    #172346

    Purpose

    Expression of FLAG-tagged, codon-optimized, truncated SRSF3

    Depositor

    Sujatha Jagannathan

    Article

    Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

    Insert

    serine and arginine rich splicing factor 3 (SRSF3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized, truncated at amino acid 114 with…

    Promoter

    EF-1a

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. LADL Bridge + Promoter Only Target

    Plasmid

    #127669

    Purpose

    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry and gRNAs 115 and 117
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and hU6

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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