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Lenti-CRISPR-V2-gYap-2

Plasmid

#166482

Purpose

Expresses spCas9 and a gRNA targeting mouse Yap

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Yap (Yap1 Mouse)

Use

Lentiviral

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A- L308E

Plasmid

#166462

Purpose

Expresses fusion of mEGFP and YAPS127A- L308E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L308E (YAP1 Human)

Expression

Mammalian

Mutation

YAPS127A- L308E

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A-deltasIDR

Plasmid

#166460

Purpose

Expresses fusion of mEGFP and YAPdeltasIDR (delta360-449)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltasIDR (YAP1 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A-deltasTAD

Plasmid

#166461

Purpose

Expresses fusion of mEGFP and YAPdeltasTAD (delta450-504)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltasTAD (YAP1 Human)

Expression

Mammalian

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry-coiled coil

Plasmid

#166448

Purpose

Expresses fusion of CRY2PHR with mCherry and YAP Coiled-coil domain

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, coiled coil(298-359) (CRY2 Human)

Use

Lentiviral

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-NF2

Plasmid

#166473

Purpose

Expresses fusion of mEGFP and NF2

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, NF2 (NF2 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A- L325E

Plasmid

#166464

Purpose

Expresses fusion of mEGFP and YAPS127A- L325E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L325E (YAP1 Human)

Expression

Mammalian

Mutation

YAPS127A- L325E

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NES-YFP-CCTd

Plasmid

#184326

Purpose

Mammalian expression of the cytosolic localized YFP-CCTd(peptide of Cav1.3)

Depositor

Xiaodong Liu

Article

Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

Insert

CCTd (CACNA1D Human)

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NLS-YFP-CCTd

Plasmid

#184325

Purpose

Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.3)

Depositor

Xiaodong Liu

Article

Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

Insert

CCTd (CACNA1D Human)

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A- L318E

Plasmid

#166465

Purpose

Expresses fusion of mEGFP and YAPS127A- L318E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L318E (YAP1 Human)

Expression

Mammalian

Mutation

YAPS127A- L318E

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-cOpn5-T2A-EGFP

Plasmid

#237858

Purpose

The transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoter

Depositor

Minmin Luo

Article

Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

Insert

Opn5 (OPN5 Chicken)

Use

AAV and Cre/Lox

Promoter

EF1α

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-cOpn5-T2A-mcherry

Plasmid

#238009

Purpose

The transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoter

Depositor

Minmin Luo

Article

Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

Insert

Opn5 (OPN5 Chicken)

Use

AAV and Cre/Lox

Promoter

EF1α

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLX313_PELO_WT

Plasmid

#228938

Purpose

Constitutive expression of wild-type human PELO

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO (PELO Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051d_sgCiPELO #2

Plasmid

#229018

Purpose

Expression of CRISPRi PELO sgRNA #2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO (PELO Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051d_sgCiPELO #1

Plasmid

#229017

Purpose

Expression of CRISPRi PELO sgRNA #1

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO (PELO Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-enCas12a-A3A-N57G

Plasmid

#193651

Purpose

Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-hA3A-N57G, EGFP

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-RR-A3A-N57G

Plasmid

#193654

Purpose

Expression of base editor RR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

RR-dLbCas12a-hA3A-N57G, EGFP

Expression

Mammalian

Mutation

dLbCas12a-G532R/K595R, hAPOBEC3A-N57G

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-RR-A3A-Y130F

Plasmid

#193655

Purpose

Expression of base editor RR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

RR-dLbCas12a-hA3A-Y130F, EGFP

Expression

Mammalian

Mutation

dLbCas12a-G532R/K595R, hAPOBEC3A-Y130F

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ND1-W86-R17-G1333N-UGI-Puro

Plasmid

#198851

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND1-W86-R17 TALEN

Expression

Mammalian

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ND6-W82-R15-G1333C-UGI-Puro

Plasmid

#198861

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND6-W82-R15 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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