We narrowed to 13,007 results for: cDNA

1. pcDNA3-Venus-CAAX

   Plasmid

   #87612

   Purpose

   Plasma membrane-targeted Venus (YFP).

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   Venus-CAAX
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-GFP-p48

   Plasmid

   #11616

   Depositor

   Steven Johnson

   Insert

   Interferon-Stimulated Gene Factor 3 (IRF9 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-BaLgp140-8his

   Plasmid

   #100924

   Purpose

   Mammalian expression plasmid for soluble gp140 from the BaL HIV-1 strain

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BaL) Env
   

   Use

   Tags

   CD5 leader sequence and Purification tag after D6…

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. hE-cadherin/Δβ-catenin-pcDNA3

   Plasmid

   #45772

   Depositor

   Barry Gumbiner

   Article

   Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

   Insert

   hE-cadherin/Δβ-catenin (CDH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ends at amino acid 844. Last 35 amino acids dele…

   Promoter

   CMV

   Available since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_mGFP-FGFR3-K650Q

   Plasmid

   #191756

   Purpose

   Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   c.1948A>C (p.K650Q) + silent mutation c.1938C&…

   Promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. NLS-4LNDD-pcDNA3.1(+)

   Plasmid

   #172313

   Purpose

   Expresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cells

   Depositor

   Joshua Rosenthal

   Article

   Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

   Insert

   Lambda N peptide - human ADAR2 Deaminase Domain
   

   Use

   Tags

   6xHis, Flag, and Nuclear Localization Signal of S…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-TAT-2-84-FLAG

   Plasmid

   #35980

   Depositor

   Susana Valente

   Article

   Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

   Insert

   transactivating protein (tat )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   tat from HIV2 84 amino acids

   Promoter

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ALK7 K222R

   Plasmid

   #80887

   Purpose

   mammalian expression of ALK7 K222R

   Depositor

   Aristidis Moustakas

   Article

   Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

   Insert

   ALK7 (Acvr1c Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K222R

   Promoter

   CMV

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/tom20-pbira(79-c)-ha

   Plasmid

   #154456

   Purpose

   expresses tom20-pbira(79-c)-ha/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   tom20-pbira(79-c)-ha (TOMM20 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA-EglN3 H196A-pcDNA3

    Plasmid

    #22717

    Depositor

    William Kaelin

    Article

    Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

    Insert

    Egg laying Nine - 3 (EGLN3 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    mutant cDNA: point mutation at codon 196 was ins…

    Promoter

    Available since

    Dec. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3puro-mGFP-ROCK2

    Plasmid

    #225472

    Purpose

    Express mEGFP-fusion protein of ROCK2

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    ROCK2 (ROCK2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcdna3 flag - ULK1 wt

    Plasmid

    #27636

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse ULK1 (Ulk1 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Initial M from ULK1 removed.

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-N-HA-NEK7 K64M

    Plasmid

    #75143

    Purpose

    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lys 64 mutated to Met; kinase inactive and F236L …

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOpen-SfiIR (cDNA)

    Plasmid

    #165528

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    SfiI (used to create cDNA libraries)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pcDNA3 barr1 flag

    Plasmid

    #14687

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    beta arrestin 1 (Arrb1 Rat)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.2-DEST-MatryoshCaMP6s

    Plasmid

    #100025

    Purpose

    Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    MatryoshCaMP6s
    

    Use

    Tags

    V5 Epitope Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Lyn-PlcR

    Plasmid

    #181830

    Purpose

    Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-PlcR
    

    Use

    Tags

    ECFP, EYFP, and N-terminal targeting sequence fro…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO V5 HSPA6

    Plasmid

    #19513

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 6 (HSPA6 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-FLAG-hDIXDC1 WT

    Plasmid

    #61222

    Purpose

    Mammalian expression vector encoding long isoform of human DIXDC1

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    human DIXDC1 (DIXDC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

    Plasmid

    #175534

    Purpose

    Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag7-myc_PDGFRtmb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge