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pBzCas13b-gRNA-3

Plasmid

#164860

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-3

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pT7gRNA-ccdb

Plasmid

#140864

Purpose

Backbone for making sgRNAs.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb/CAM

Use

Gateway destination vector

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-639 (attR4-attR2)

Plasmid

#161880

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-624 (attR4-attR2)

Plasmid

#161879

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-HypaCas9-D1135E

Plasmid

#154930

Purpose

aTc inducible HypaCas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

HypaCas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

N692A/M694A/Q695A/H698A/D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-D1135E

Plasmid

#154924

Purpose

aTc inducible Cas9HF1 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

Cas9HF1-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A/D1135E and stop codon on …

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0548

Plasmid

#162299

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-TrxA-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEE22

Plasmid

#161787

Purpose

Hi-SC2 reporter delivery plasmid

Depositor

François-Xavier Barre

Article

Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. d

Insert

Hi-SC2 reporter

Use

Conjugative plasmid to deliver hi-sc2 reporter

Mutation

Himar site included MmeI site, tandem loxP sites,…

Promoter

n/a

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0545

Plasmid

#162296

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-MBP-[Factor Xa cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0542

Plasmid

#162293

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-GST-[thrombin cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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