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Plasmid#80394PurposeContains aTc inducible Scaffold-mCherry-AAV and TetR genesDepositorInsertmCherry RFP
UseSynthetic BiologyTagsFused by GS linkers to Sh3pep-LZX scaffold protei…ExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKS013
Plasmid#65466PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing mCherryDepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpl-TetOAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL 309
Plasmid#64906PurposeKanR + ColE1 + PLlacO-1::RBS(st7):rfp::RBS(st7):cfp::RBS(st7):yfp::T1 terminatorDepositorTypeEmpty backboneUseSynthetic Biology; E. coliTagsExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
ART
Plasmid#214063PurposeFluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ARTi
Plasmid#214064PurposeFluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ARTX_ctrl
Plasmid#214065PurposeFluorescent reporter system, NMD negative control. No intron in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ART_RTctrl
Plasmid#214066PurposeFluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM2s2TsR
Plasmid#137923PurposeE. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM1s3TsR
Plasmid#137922PurposeE. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDL278_P23-DsRed-Express2
Plasmid#121505PurposeE. coli-streptococcal shuttle vector that expresses rfpDepositorInsertDsRed-Express2
UseE. coli streptococcal shuttle vectorTagsExpressionMutationPromoterP23Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTdTomato-L5
Plasmid#140994PurposetdTomato, StrepR, L5int, attP to create RFP mycobacteriaDepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterhsp60Available sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
AiRTi
Plasmid#214068PurposeFluorescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiRTX_ctrl
Plasmid#214069PurposeFluorescent reporter system, NMD negative control. Intron present in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiRT
Plasmid#214067PurposeFluorescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiRT_RTctrl
Plasmid#214070PurposeFluorescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL11015
Plasmid#117499PurposeFAST-Red selectable marker Golden Gate Level 1 Position 1DepositorInsertBpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
UseTagsRFPExpressionPlantMutationPromoterAtOLE1Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL50004
Plasmid#50316DepositorInsertCDS, mCherry variant of RFP (Discosoma sp.)
UseTagsExpressionPlantMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSECRETS-A
Plasmid#196986PurposeFor SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpltetO1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
BASIC_8_RBS34- mCherry -B0015
Plasmid#68142Purposestorage vector for BASIC part: RBS-RFP(ORF)DepositorInsertRBS34-mcherry-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL80007
Plasmid#50323DepositorInsertCDS, mCherry variant of RFP (Discosoma sp.)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only