We narrowed to 18,057 results for: puro

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pCLJ-P2F-Frt-PGK-Puro-Frt

Plasmid

#227501

Purpose

Inserts 500bp Fgf5 promoter in place of the Prdm8 promoter along with removable selection cassette

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Fgf5 promoter

Use

AAV and Mouse Targeting

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro GFP-REX1-IRES-mCherry

Plasmid

#232239

Purpose

Mammalian expression of REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Tags

AcGFP1

Expression

Mammalian

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPD1178-LPDV promoterless PuroR-P2A-miRFP720

Plasmid

#201537

Purpose

Landing pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 gene

Depositor

Joshua Leonard

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

Promoterless PuroR-P2A-miRFP720

Expression

Mammalian

Promoter

None

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-shRNA2-zsGreen-PGK-puro_HSALNG0104472-163

Plasmid

#197902

Purpose

For the stable knockdown experiments using shRNA, a complementary sequence (HSALNG0104472-163) targeting the HSALNG0104472 transcript was inserted.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

HSALNG0104472-163 (HSALNG0104472-scramble-sequence)

Use

Lentiviral

Tags

zsGreen1

Expression

Mammalian

Mutation

15q11.2 deletion

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse STING #1-gRNA-Cas9-Puro

Plasmid

#186892

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse STING #2-gRNA-Cas9-Puro

Plasmid

#186893

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-N1-KH1mut (T236D H237D)

Plasmid

#225453

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (T236D H237D)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

T236D H237D

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A4

Plasmid

#216823

Purpose

Expresses acyclovir-controllable IRE variant #A4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A4

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8

Plasmid

#216824

Purpose

Expresses acyclovir-controllable IRE variant #A8 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-3

Plasmid

#216825

Purpose

Expresses acyclovir-controllable IRE variant #A8-3 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8-3

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-4

Plasmid

#216826

Purpose

Expresses acyclovir-controllable IRE variant #A8-4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8-4

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-ChloC

Plasmid

#179841

Purpose

donor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

chloride-conducting ChR (ChloC)

Expression

Mammalian

Promoter

Troponin T promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-iChloC

Plasmid

#179842

Purpose

donor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

improved chloride-conducting ChR (iChloC)

Expression

Mammalian

Promoter

Troponin T promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-luciferase

Plasmid

#179844

Purpose

donor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR, Luciferase, and TALEN ; Donor plasmid

Tags

luciferase

Expression

Mammalian

Promoter

Troponin T promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-mCherry-RAB7-S72A

Plasmid

#221556

Purpose

Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Mutation

Change serine 72 to alanine

Promoter

EF1a

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-IRES-puro6 +gal4DBDHIFCAD pGalO linker

Plasmid

#207172

Purpose

expression plasmid for Gal4DBD-HIF-CAD expression GalO linker

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD GalO linker

Expression

Mammalian

Promoter

EF1a

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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SOX2-P2A-NLS-mClover3-IRES-Puro

Plasmid

#215544

Purpose

Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus

Depositor

Kai Wang

Insert

SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)

Use

CRISPR

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 Cdk1mut7A-C-Myc-P2A-Puro

Plasmid

#211617

Purpose

Rhno1 Cdk1mut7A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Expression

Mammalian

Mutation

S58A, S98A, S133A, T159A, T198A, S191A, and T202A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(12))-PGKpuroBFP-W

Plasmid

#208569

Purpose

Lentiviral vector expressing gRNA targeting human RUNX1 and RUNX3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RUNX1(13), RUNX3(12) (RUNX1, RUNX3 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(13))-PGKpuroBFP-W

Plasmid

#208570

Purpose

Lentiviral vector expressing gRNA targeting human RUNX1 and RUNX3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RUNX1(13), RUNX3(13) (RUNX1, RUNX3 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(21))-PGKpuroBFP-W

Plasmid

#200507

Purpose

Lentiviral vector expressing gRNA targeting human ARID1A and ARID1B

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1A(44), ARID1B(21) (ARID1A, ARID1B Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

Plasmid

#200506

Purpose

Lentiviral vector expressing gRNA targeting human ARID1A and AAVS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1A(44), AAVS1 (ARID1A, AAVS1 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(23))-PGKpuroBFP-W

Plasmid

#200508

Purpose

Lentiviral vector expressing gRNA targeting human ARID1A and ARID1B

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1A(44), ARID1B(23) (ARID1A, ARID1B Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(AAVS1)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#200502

Purpose

Lentiviral vector expressing gRNA targeting human AAVS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

AAVS1 (AAVS1 Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

Plasmid

#200503

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(43), AAVS1 (SMARCA4, AAVS1 Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

Plasmid

#189633

Purpose

Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(47))-PGKpuroBFP-W

Plasmid

#200505

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(43), SMARCA2(47) (SMARCA4, SMARCA2 Human)

Use

Lentiviral

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro

Plasmid

#189634

Purpose

Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

hU6 Cas9-Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

Plasmid

#189632

Purpose

Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TetOne-Puro-p62 K7A/D69A

Plasmid

#204549

Purpose

Expresses p62 K7A/D69A in a doxycycline-dependent manner in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K7A/D69A

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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