We narrowed to 5,601 results for: Feng Zhang

1. PP7 guide

   Plasmid

   #86168

   Purpose

   Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   PP7 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

   Plasmid

   #196710

   Purpose

   Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AiP942-pX330-Sncg

   Plasmid

   #181774

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Sncg guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AiP926-pX330-Batf3

   Plasmid

   #181772

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Batf3 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AiP927-pX330-Chrna6

   Plasmid

   #181765

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Chrna6 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AiP928-pX330-Gpr139

   Plasmid

   #181767

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Gpr139 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AiP938-pX330-Slc17a7

   Plasmid

   #181775

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Slc17a7 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AiP939-pX330-Ctxn3

   Plasmid

   #181769

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Ctxn3 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBoBi-hLAMP2-C-GC6s

   Plasmid

   #154151

   Purpose

   To detect the calcium ion releases close to the lysosomal surface

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

   Insert

   LAMP2 and GCamp6s (LAMP2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LentiCRISPRv2 RFP670

    Plasmid

    #187646

    Purpose

    lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site

    Depositor

    Martin Carroll

    Insert

    RFP 670
    

    Use

    Lentiviral

    Tags

    RFP670 and sgRNA cloning site

    Expression

    Mutation

    Promoter

    EFS (P2A)

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. eSpCas9(1.1)-T2A-Puro

    Plasmid

    #101039

    Purpose

    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

    Depositor

    Andrea Németh

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFLAG and T2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti_P2A_Blast

    Plasmid

    #211365

    Purpose

    Lentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfections

    Depositor

    Jacques Neefjes

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV8R-12 Capsid

    Plasmid

    #213968

    Purpose

    AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence

    Depositor

    Zhonghua Lu

    Article

    Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223249

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PA-Dre

    Plasmid

    #169706

    Purpose

    Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag-T2A-pMag-Dre247
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCherry-pCAG-donor(R2Tg)-GFP(intron)

    Plasmid

    #223248

    Purpose

    Mammalian expression of the R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-pCALM1-EYFP-WPRE-bGHpA

    Plasmid

    #213971

    Purpose

    AAV expression of EYFP from pCALM1 promoter

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    pCALM1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAVS1-pur-CAG-Bi-DREADD

    Plasmid

    #159457

    Purpose

    AAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection gene

    Depositor

    Yuejun Chen

    Article

    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

    Insert

    Bi-DREADD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fusion protein of hM3Dq-mCherry and HA-KORD seper…

    Promoter

    CAG

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213973

    Purpose

    AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-hsyn-GRAB_rDA-mut

    Plasmid

    #140558

    Purpose

    Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-hsyn-GRAB_DA-mut

    Plasmid

    #140555

    Purpose

    Expresses the DA-insensitive control sensor GRAB_DA-mut in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    DA-insensitive sensor GRAB_DA-mut (control sensor)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC19_TELO2_dTAG

    Plasmid

    #224380

    Purpose

    Generates TELO2 dTAG N terminal knock-in cell lines

    Depositor

    Junjie Chen

    Article

    Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

    Insert

    TELO2 (TELO2 Human)
    

    Use

    Tags

    dTAG

    Expression

    Bacterial

    Mutation

    Synonymous mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUC19_GNB1L_dTAG

    Plasmid

    #224378

    Purpose

    Generates GNB1L dTAG C terminal knock-in cell lines

    Depositor

    Junjie Chen

    Article

    Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

    Insert

    GNB1L (GNB1L Human)
    

    Use

    Tags

    dTAG

    Expression

    Bacterial

    Mutation

    Synonymous mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

    Plasmid

    #194750

    Purpose

    To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti_CMV_Puro_SFB_GNB1L

    Plasmid

    #224381

    Purpose

    Lenti plasmid for generating GNB1L expressing stable cell lines

    Depositor

    Junjie Chen

    Article

    Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

    Insert

    GNB1L (GNB1L Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX459_TELO2_gRNA

    Plasmid

    #224379

    Purpose

    Targets TELO2 for dTAG N terminal knock-in cell lines

    Depositor

    Junjie Chen

    Article

    Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

    Insert

    TELO2 (TELO2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS

    Plasmid

    #194751

    Purpose

    To fuse your protein of interest to the C-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PECAM1-3'gRNA

    Plasmid

    #235606

    Purpose

    gRNA targeting PECAM1 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al Angiogenesis. 2025 Jan 3;28(1):10. doi:

    Insert

    PECAM1 gRNA (PECAM1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ER50-SpCas9-ER50

    Plasmid

    #85448

    Purpose

    Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    ER50-SpCas9-ER50
    

    Use

    AAV

    Tags

    destabilized domain of estrogen receptor ligand b…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. lenti-Cas9-sgHPRT1

    Plasmid

    #196713

    Purpose

    CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Insert

    Cas9-T2A-BSD-U6-sgHPRT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a/hU6

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

    Plasmid

    #175571

    Purpose

    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. lenti-dCas9-VP64-BFP

    Plasmid

    #196711

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-VP64-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

    Plasmid

    #175572

    Purpose

    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. lenti-UCOE-dCas9-BFP-VP64

    Plasmid

    #196717

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. lentiCRISPRv2-sgCTL

    Plasmid

    #234771

    Purpose

    Non Targeting Control

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Non Targeting Control sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEF6-CSPG4-myc-his

    Plasmid

    #69037

    Purpose

    Expression of CSPG4 in mammalian cells

    Depositor

    Wensheng Wei

    Article

    Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

    Insert

    CSPG4 (CSPG4 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1 alpha

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-G88P7-rM3Ds-2A-EYFP

    Plasmid

    #213970

    Purpose

    AAV vector with G88P7 promoter in order to induce robust expression of rM3Ds in MSNs

    Depositor

    Zhonghua Lu

    Article

    Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

    Insert

    rM3Ds
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    G88P7

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213969

    Purpose

    AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAV-GFAP-NLS-CasRx-NLS-FLAG

    Plasmid

    #154000

    Purpose

    Express CasRx for AAV package

    Depositor

    Hui Yang

    Article

    Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. do

    Insert

    CasRx
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    GFAP

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge