We narrowed to 5,715 results for: Feng Zhang
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Plasmid#210808PurposeTo block the formation of the initial Schiff base with FBPDepositorInsertALDOA (ALDOA Human)
UseLentiviralTagsHAMutationresidues 230 of ALDOA mutated to alaninePromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT22UbGfpnE4PCh(#301)
Plasmid#184066Purposecyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenicDepositorInserteGFP-nls-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)MutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-EGFP
Plasmid#107721PurposeFor expression of sgRNA from the U6 promoter with EGFP expression simultaneouslyDepositorTypeEmpty backboneUseCRISPRAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-GFP-Fis1
Plasmid#182576PurposeExpresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertLifeAct-GFP-Fis1
TagsFis1 and LifeActExpressionMammalianAvailable SinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-GFP-cytoB5RR
Plasmid#182577PurposeExpresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequenceDepositorInsertLifeAct-GFP-cytoB5RR
TagsLifeAct and cytoB5RRExpressionMammalianAvailable SinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Fis1
Plasmid#182580PurposeExpresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertmCherry-Fis1
TagsFis1ExpressionMammalianAvailable SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-AGG-SpCas9
Plasmid#214603Purposeencode SpCas9; in vitro transcribed modified RNA vectorDepositorInsertSpCas9
UseCRISPR and UnspecifiedPromoterT7Available SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-BFP
Plasmid#107722PurposeFor expression of sgRNA from the U6 promoter with BFP expression simultaneouslyDepositorTypeEmpty backboneUseCRISPRAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS human IRS-1
Plasmid#11359DepositorInsertIRS-1 (IRS1 Human)
ExpressionBacterialAvailable SinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Sa sgRNA expression vector
Plasmid#107720PurposeFor in vitro transcription of Sa sgRNA from the T7 promoter.DepositorTypeEmpty backboneUseCRISPRAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-AGG-NLS-EGFP
Plasmid#214602Purposeexpress EGFP ; in vitro transcribed modified RNA vectorDepositorInsertEGFP
UseUnspecifiedPromoterT7Available SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hAP1-C-LAMP2 TM
Plasmid#183645PurposeLentiviral expression of a mutant of ATP6AP1 with its PEN2-interacting domain replaced with the transmembrane (TM) domain of LAMP2.DepositorUseLentiviralTagsHAMutationATP6AP1 transmembrane domain replaced by the tran…PromoterCMVAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
ACBE-Q
Plasmid#204608PurposeMammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-QDepositorInsertbpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationTadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …Available SinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-Q2L7-T2176N-UGI-Puro
Plasmid#209647PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-Q2L7-T2176N
ExpressionMammalianAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-Q2L7-T2176C-UGI-Puro
Plasmid#209646PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-Q2L7-T2176C
ExpressionMammalianAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-FZY2-S100N-UGI-Puro
Plasmid#209642PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-FZY2-S100N
ExpressionMammalianAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-FZY2-S100C-UGI-Puro
Plasmid#209641PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-FZY2-S100C
ExpressionMammalianAvailable SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-Q2L7-T2176N-UGI-Puro
Plasmid#209649PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-Q2L7-T2176N
ExpressionMammalianAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKnockinDonor-humanCTCF-miniAID-mClover3
Plasmid#136883Purposeknockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCFDepositorInsertCTCF-knockin-homology arm-miniAID-mClover3
TagsminiAID-mClover3ExpressionMammalianPromoterno promoterAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-Q2L7-T2176C-UGI-Puro
Plasmid#209648PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-Q2L7-T2176C
ExpressionMammalianAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-FZY2-S100N-UGI-Puro
Plasmid#209644PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-FZY2-S100N
ExpressionMammalianAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-FZY2-S100C-UGI-Puro
Plasmid#209643PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-FZY2-S100C
ExpressionMammalianAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2iApowriter2(#336)
Plasmid#184104Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesisTagsinternal tag: 5mycMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2iU6Apowriter2N(#355)
Plasmid#184106Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesisDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesis + in vitro trans…Tagsinternal tag: 5mycMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-Ubi+SP6Available SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22Apowriter2(#340)
Plasmid#184105Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)Tagsinternal tag: 5mycMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22iuasvApoWtEos3N(#486)
Plasmid#184107Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)MutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …Promoter4xUASAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22iuasvApoWChe(#488)
Plasmid#184108Purposecyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)MutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …Promoter4xUASAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1
Plasmid#125840Purposeexpress wild type BAP1DepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-C91A
Plasmid#125841Purposeexpress mutant BAP1-C91ADepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-H169Q
Plasmid#125847Purposeexpress mutant BAP1-H169QDepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-C91G
Plasmid#125845Purposeexpress mutant BAP1-C91GDepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-G185R
Plasmid#125849Purposeexpress mutant BAP1-G185RDepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA D34S
Plasmid#210810PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
TagsHAExpressionMammalianMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-E31A
Plasmid#125843Purposeexpress mutant BAP1-E31ADepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-S10T
Plasmid#125842Purposeexpress mutant BAP1-S10TDepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-L49V
Plasmid#125844Purposeexpress mutant BAP1-L49VDepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-F170C
Plasmid#125848Purposeexpress mutant BAP1-F170CDepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-G109V
Plasmid#125846Purposeexpress mutant BAP1-G109VDepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-hALDOA D34S
Plasmid#210813PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
UseAdenoviralTagsHAMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hALDOA D34S
Plasmid#210807PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
UseLentiviralTagsHAMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only