We narrowed to 9,300 results for: bli

1. PAS_RvSAHS1

   Plasmid

   #215645

   Purpose

   Expresses SUMO-tagged RvSAHS1 protein from tardigrade R. varieornatus in E. coli

   Depositor

   Pamela Silver

   Insert

   SAHS1
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SIRT6

   Plasmid

   #41565

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SIRT6 (SIRT6 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   aa 3-353

   Promoter

   T7

   Available Since

   April 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pAG426-GAL-UBQLN2

   Plasmid

   #181708

   Purpose

   Galactose inducible expression of UBQLN2

   Depositor

   Alejandro Chavez

   Insert

   UBQLN2 (UBQLN2 Human)
   

   Expression

   Yeast

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_RapACR_T111C_N248Q-EYFP

   Plasmid

   #119197

   Purpose

   Mammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248Q

   Depositor

   John Spudich

   Insert

   Anion channelrhodopsin RapACR fast mutant T111C_N248Q
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed threonine 111 to cysteine and asparagine …

   Promoter

   CMV

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

   Plasmid

   #185060

   Purpose

   Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445

   Depositor

   Bobby Koeleman

   Insert

   U6:pegRNA:scaffold:PBS+RT template
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-synP-FLEX-TVA66T-EGFP-B19G

   Plasmid

   #64097

   Purpose

   Expresses TVA66T-EGFP-B19G under the synapsin promotor

   Depositor

   Ian Wickersham

   Insert

   TVA66T-EGFP-B19G
   

   Use

   AAV and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Glutamic acid 66 to Threonine

   Promoter

   synapsin

   Available Since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. PTPN7

   Plasmid

   #38945

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PTPN7 (PTPN7 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. TMPRSS2 g1 + Cas9

   Plasmid

   #153015

   Purpose

   A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + TMPRSS2 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP2

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-FLEX-SaCas9-U6-sgSlc6a3

   Plasmid

   #159902

   Purpose

   Mutagenesis of Slc6a3

   Depositor

   Larry Zweifel

   Insert

   Slc6a3 (Slc6a3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons

    Plasmid

    #172479

    Purpose

    tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-7 asyn S87E/S129E

    Plasmid

    #36064

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein S87E/S129E (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    S87E/S129E

    Promoter

    T7

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. PHGDH

    Plasmid

    #38841

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PHGDH (PHGDH Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pGL3-IL-4

    Plasmid

    #40570

    Depositor

    Alexander Dent, Mark Kaplan

    Insert

    IL-4 promoter (Il4 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    contains 250 bp of the proximal mouse IL-4 promot…

    Promoter

    mouse IL-4

    Available Since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

14. TLCV2 sgAAVS1

    Plasmid

    #127099

    Purpose

    Encodes gRNA for human AAVS1.

    Depositor

    Adam Karpf

    Insert

    AAVS1 (PPP1R12C AAVS1 Human) (PPP1R12C Human)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. hMYF5-3xHA-IRES-hrGFP (#397)

    Plasmid

    #78331

    Purpose

    Overexpression of human MYF5 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYF5 (MYF5 Human)
    

    Tags

    3x HA tag and IRES-hrGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pSpCas9_mouse_Adar1_ccA

    Plasmid

    #158120

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_hSMAD4

    Plasmid

    #212710

    Purpose

    The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.

    Depositor

    Catharine Ross, Reza Zolfaghari

    Insert

    Homo sapiens SMAD4 (SMAD4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZC03 (pFA6a-TEV-6xGly-V5-His3MX)

    Plasmid

    #44072

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, His3…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pINDUCER20-hMYF5-3xHA (#732)

    Plasmid

    #78332

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a 3xHA tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYF5 (MYF5 Human)
    

    Use

    Lentiviral

    Tags

    3x HA tag

    Promoter

    TRE2

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-FLAG-DDB1 (1140H)

    Plasmid

    #174849

    Purpose

    Expression of DDB1

    Depositor

    Helen Morrison

    Insert

    DDB1 (DDB1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pTF264 (pFA6a-TEV-6xGly-3xFlag-His3MX)

    Plasmid

    #44071

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    3xFlag, 6xGly linker, ADH1 transcription terminat…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSMART-Mev1

    Plasmid

    #65815

    Purpose

    NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Inserts

    mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
    mvaS: hydroxymethylglutaryl-CoA synthase
    

    Expression

    Bacterial

    Promoter

    insulated mipAp from E. coli and insulated waaHp…

    Available Since

    Sept. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLEX307-TMPRSS4-G418

    Plasmid

    #158474

    Purpose

    Constitutive lentiviral expression of human TMPRSS4.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    TMPRSS4 (TMPRSS4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid

    #104413

    Purpose

    transient expression of dCas9-dTET1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dTET1
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H1672Y-D1674A

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTF270 (pFA6a-PSc-6xGly-3xFlag-KanMX)

    Plasmid

    #44089

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    3xFlag, 6xGly linker, ADH1 transcription terminat…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MAGEA4

    Plasmid

    #39001

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAGEA4 (MAGEA4 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFR022

    Plasmid

    #134228

    Purpose

    bacterial expression of KNL1

    Depositor

    Iain Cheeseman

    Insert

    hKNL1 aa 2106-2316 (KNL1 Human)
    

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBABEpuro-CRAF_S259A

    Plasmid

    #51127

    Purpose

    mammalian expression of wt CRAF S259A

    Depositor

    Matthew Meyerson

    Insert

    CRAF (RAF1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    S259A

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PTPRN2

    Plasmid

    #38891

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPRN2 (PTPRN2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

30. 2P0E

    Plasmid

    #60109

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    C9orf95:C9orf95_dml-X01:C201158 (NMRK1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

31. PRDM11

    Plasmid

    #32858

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PRDM11 (PRDM11 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 79-314

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

32. RPS6KA1

    Plasmid

    #39021

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RPS6KA1 (RPS6KA1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

33. MIGR1-Meis1a

    Plasmid

    #78148

    Purpose

    retroviral expression of Meis1a and GFP

    Depositor

    Francesco Blasi

    Insert

    Meis1a (Meis1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

34. p223-RV-dlx-dio-Tdtomato-T2A-SYPEGFP

    Plasmid

    #185699

    Purpose

    Retroviral vector expression cre-dependent Tdtomato-T2A-SynaptophysinEGFP driven by the mDlx promoter

    Depositor

    Lynette Lim

    Inserts

    Tdtomato,
    Synaptophysin-EGFP
    

    Use

    Cre/Lox and Retroviral

    Tags

    EGFP and T2A

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SETD3

    Plasmid

    #32873

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SETD3 (SETD3 Human)
    

    Tags

    His tag (with TEV cleavage)

    Expression

    Bacterial

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

36. pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid

    #53206

    Purpose

    N-terminal chromosomal tagging with stable GFP under control of GAL promoter

    Depositor

    John Pringle

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP (F64L, S65T, V163A) and TRP1

    Promoter

    GAL1 promoter

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pSBtet-Bla/PAG1-GFP

    Plasmid

    #198220

    Purpose

    Transposon vector (Addgene #60510) for doxycycline-inducible expression of human PAG1 fused to EGFP

    Depositor

    Eric Davis

    Insert

    PAG1 (PAG1 Human)
    

    Use

    Transposon

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    None

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HaloTag-G protein gamma-7

    Plasmid

    #67040

    Purpose

    G protein gamma-7 tagged with HaloTag on amino terminus

    Depositor

    Catherine Berlot

    Insert

    HaloTag-G protein gamma-7 (GNG7 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCB32

    Plasmid

    #111911

    Purpose

    Expresses gRNA to target Nte1 locus and has HygR marker

    Depositor

    Yi Tang

    Insert

    gRNA cassette targeting Nte1 locus
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    SNR52p

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKG210

    Plasmid

    #69761

    Purpose

    Retroviral expression of CENP-T aa 1-375

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-375 (CENPT Human)
    

    Use

    Retroviral

    Tags

    LacI-NLS and mCherry

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only