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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,066 results for: CAMP

Showing: 2201 - 2220 of 3066 results

  1. pcDNA3.1-VcnCS-E1015A-E1021A

    Plasmid
    #213414
    Purpose
    Vinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnCS-E1015A-E1021A (VCL Synthetic, Chicken)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1015 and 1021 to a…
    Promoter
    CMV
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213414-plasmid-map-sequence-id-422048

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  3. pHW582

    Plasmid
    #198819
    Purpose
    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, higher baseline fluorescence
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::GCaMP7b-SL2-mKate2::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    198819-plasmid-map-sequence-id-390987

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  4. pHW580

    Plasmid
    #198816
    Purpose
    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, slow kinetics
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::GCaMP7s-SL2-mKate2::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    198816-plasmid-map-sequence-id-390989

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  5. iDianiSnFR

    Plasmid
    #177741
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iDianiSnFR
    Use
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177741-plasmid-map-sequence-id-348683

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  6. iCytSnFR

    Plasmid
    #177738
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCytSnFR
    Use
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    177738-plasmid-map-sequence-id-348679

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  7. iCyt_BrEt_SnFR

    Plasmid
    #177740
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCyt_BrEt_SnFR
    Use
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    177740-plasmid-map-sequence-id-349387

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  8. iCyt_F_SnFR

    Plasmid
    #177739
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCyt_F_SnFR
    Use
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    177739-plasmid-map-sequence-id-348682

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  9. pSMT3-FBF2 SS/R

    Plasmid
    #166976
    Purpose
    Expresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Traci Hall
    Article
    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1
    Insert
    FBF-2 (fbf-2 Nematode)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166976-plasmid-map-sequence-id-331635

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  10. pSMT3-FBF2 AQ/Y

    Plasmid
    #166975
    Purpose
    Expresses FBF-2 with AQ/Y mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Traci Hall
    Article
    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1
    Insert
    FBF-2 (fbf-2 Nematode)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166975-plasmid-map-sequence-id-331630

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  12. pRSM4

    Plasmid
    #101642
    Purpose
    Encodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.
    Depositor
    Thomas Hughes
    Article
    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548
    Insert
    KCyG4219
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    C-terminal residues (221-223) deletion. Ser220Val…
    Promoter
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    101642-plasmid-map-sequence-id-195172

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  20. pAAV2-CAG-mGreenLantern-KASH

    Plasmid
    #191094
    Purpose
    To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Pantelis Tsoulfas, Murray Blackmore
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    mGreenLantern-KASH
    Use
    AAV and Synthetic Biology
    Tags
    mGreenLantern fused to the KASH domain of Nesprin…
    Expression
    Mammalian
    Mutation
    Optimizzation was done using the Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191094-plasmid-map-sequence-id-380125

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Showing: 2201 - 2220 of 3066 results