We narrowed to 16,337 results for: LEA

Showing: 2201 - 2220 of 16337 results

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pNA 3C myc scfvHA

Plasmid

#234991

Purpose

Expression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteins

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

NA-3C-myc-scfvHA

Expression

Mammalian

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

Plasmid

#61629

Purpose

R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

Depositor

Dolf Weijers

Article

Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

Inserts

mDII
ntdTomato
DII
n3xVenus

Expression

Plant

Promoter

RPS5A

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pDonor_ISCro4_(WT)_Donor_ECFP

Plasmid

#251501

Purpose

Donor plasmid for monitoring insertion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL and Tol2_ISCro4_Insertion_Reporter plasmid

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4_(WT)_Donor sequence and ECFP

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

Plasmid

#86674

Purpose

Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

GFP-FLAG-cGAS E225A/D227A

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mClover3-Puromycin

Plasmid

#167205

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mClover3

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mRuby3-Zeo

Plasmid

#167206

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mRuby3

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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AP4mS_PPVs_P3_cLuc

Plasmid

#119300

Purpose

Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Cyto-mTurq2-Apollo-NADP+

Plasmid

#193301

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasm

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Tags

mTurqoise2

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-ExRai-AktAR2

Plasmid

#184049

Purpose

Nuclear-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Nuc-ExRai-AktAR2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A RIGI WT

Plasmid

#167289

Purpose

Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

DDX58 (RIGI Human)

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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nesprin TSmod 25

Plasmid

#125555

Purpose

Nesprin-2G force sensor with cerulean-F25-venus TSmodule

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

mini-nesprin-2G (Syne2 Mouse)

Tags

cerulean, venus

Expression

Mammalian

Mutation

TSmod changed from mTFP to cerulean, flageliform …

Promoter

CMV

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(PGK/CMV)

Plasmid

#172330

Purpose

LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

PGK/CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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LBR pEGFP-N2 (646)

Plasmid

#61996

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

LBR (LBR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pNESx2-EGFP-C1-PABDx2-Spo20

Plasmid

#220082

Purpose

PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

Spo20 (SPO20 Budding Yeast)

Tags

X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

Expression

Mammalian

Mutation

amino acids 51-91, tandem dimer

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a)-TTN

Plasmid

#172327

Purpose

LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-HaloTag-Puromycin

Plasmid

#167208

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

HaloTag

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTE4398

Plasmid

#74042

Purpose

Expresses human codon-optimized LbCpf1 and Lb crRNA.

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

Lb crRNA
LbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK3

Plasmid

#122674

Purpose

Expresses mouse PCSK3 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTE4396

Plasmid

#74041

Purpose

Expresses human codon-optimized AsCpf1 and As crRNA.

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

As crRNA
AsCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

Plasmid

#117687

Purpose

U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

Depositor

Erik Sontheimer

Article

Gao et al Methods Enzymol. 2019;616:365-383. doi:

Inserts

Kanamycin cassette
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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