We narrowed to 15,732 results for: LEA

1. pCS2 Notch1 LNG (CC→SS)-6MT

   Plasmid

   #41739

   Depositor

   Raphael Kopan

   Article

   Mumm et al Mol Cell. 2000 Feb;5(2):197-206.

   Insert

   Notch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)
   

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   Contains murine Notch-1 leader peptide (aa 1-23) …

   Available Since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

2. TTR F87E

   Plasmid

   #238075

   Purpose

   Bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

   Insert

   TTR (TTR Human)
   

   Expression

   Bacterial

   Mutation

   F87E

   Promoter

   T7

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE2-TetO-Chim-Pchi-Q61A

   Plasmid

   #231520

   Purpose

   Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

   Depositor

   Ralf Jauch

   Article

   Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

   Insert

   Pchi HMG Q61A flanked with N- and C-termini of mSox2
   

   Use

   Lentiviral

   Mutation

   Point mutation at position 61 Q to A of the HMG d…

   Available Since

   March 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-TetO-Chim-Pchi-Q61A/E64M

   Plasmid

   #231521

   Purpose

   Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

   Depositor

   Ralf Jauch

   Article

   Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

   Insert

   Pchi HMG Q61A/E64M flanked with N- and C-termini of mSox2
   

   Use

   Lentiviral

   Mutation

   Point mutations at position 61 Q to A and 64 E to…

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMBS-845

   Plasmid

   #218000

   Purpose

   Strep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavage

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   Strep-tag II
   

   Use

   Synthetic Biology

   Tags

   PreScission protease cleavage site

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBS-846

   Plasmid

   #218001

   Purpose

   3xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavage

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   3xFLAG-tag
   

   Use

   Synthetic Biology

   Tags

   PreScission protease cleavage site

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMBS-847

   Plasmid

   #218002

   Purpose

   Myc-tag fused to PreScission Protease cleavage site for protein purification and cleavage

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   Myc-tag
   

   Use

   Synthetic Biology

   Tags

   PreScission protease cleavage site

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

   Plasmid

   #218916

   Purpose

   Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
   

   Use

   Lentiviral

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. KIF13B

   Plasmid

   #163907

   Purpose

   Full length mouse KIF13B for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   kif13b (Kif13b Mouse)
   

   Tags

   Cleavable 6 x HIS tag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. KIF13B 1-584

    Plasmid

    #163908

    Purpose

    Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hy_pMT Flag-URH49 MUT

    Plasmid

    #110126

    Purpose

    Expresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    URH49 (DDX39A Human)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    4 amino acid mutations (RSFS motif changed to KSL…

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Hy_pMT Flag-Hel25E MUT

    Plasmid

    #110122

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    4 mutations (KKLN motif changed to RSFS)

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. NKP91 GFP (1034)

    Plasmid

    #62023

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    Tmem41A (TMEM41A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

    Plasmid

    #134867

    Purpose

    Expresses EGFP-tagged nuclear envelope marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

    Plasmid

    #132771

    Purpose

    Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

    Depositor

    Jan Lammerding

    Article

    Denais et al Science. 2016 Apr 15;352(6283):353-8. do

    Insert

    Cyclic GMP-AMP synthase (CGAS Human)
    

    Use

    Lentiviral

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    E225A D227A

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSN-A112C-GpA-G83I

    Plasmid

    #191239

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    

    Tags

    nuclease A from S. aureus fused to the TM domain …

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and Glycin…

    Promoter

    T7 promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNA 3C myc scfvFLAG

    Plasmid

    #234990

    Purpose

    Expression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG protein

    Depositor

    Monica Gonzalez-Magaldi, Daniel Leahy

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    NA-3C-myc-scfvFLAG
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMIG-hNFATc2

    Plasmid

    #74050

    Purpose

    retroviral expression plasmid for human NFATc2/C

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc2, isoform C (NFATC2 Human)
    

    Use

    Retroviral

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    silent mutation A1723C in the sequence of NM_1730…

    Promoter

    pMSCV-LTRs

    Available Since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. FASTHDR-tRNApromo-sgRNA-espCas91_1

    Plasmid

    #167210

    Purpose

    Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid

    #167288

    Purpose

    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    APEX2 and EGFP

    Expression

    Mammalian

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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