We narrowed to 30,195 results for: REP

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pJJF496_dpy-10_CDS_sg6

Plasmid

#164267

Purpose

sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting the coding sequence of dpy-10

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF495_dpy-10_CDS_sg6

Plasmid

#164268

Purpose

sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting the coding sequence of dpy-10

Use

CRISPR

Expression

Worm

Promoter

U6 promoter from W05B2.8

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF449_dpy-10_CDS_sg1

Plasmid

#163866

Purpose

sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting the coding sequence of dpy-10

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF328_sqt-3_3′UTR_sg2

Plasmid

#163867

Purpose

sgRNA targeting sqt-3 (3′UTR) in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting the 3'UTR of sqt-3

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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S1_AscI_V5-gateway_Bsu36I

Plasmid

#128467

Purpose

destination vector with N-terminal V5 tag

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

destination vector with N-terminal V5 tag

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S2_AscI_HA-gateway_Bsu36I

Plasmid

#128468

Purpose

destination vector with N-terminal HA tag

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

destination vector with N-terminal HA tag

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC28-BSA4-S15

Plasmid

#127821

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-S10

Plasmid

#127829

Purpose

Production of recombinant human ribosomal protein S10

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS10 (RPS10 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_W22HDHFR_WTTS

Plasmid

#91241

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

W22H

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_F31VDHFR_WTTS

Plasmid

#91227

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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kank1a_pJet1.2

Plasmid

#121978

Purpose

for generating kank1a riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1a (si:dkey-146l2.1 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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MtMscL delta49

Plasmid

#118342

Purpose

M. tuberculosis MscL (delta49) in pET19b

Depositor

Douglas Rees

Article

Herrera et al Sci Rep. 2018 Oct 1;8(1):14566. doi: 10.

Insert

MscL (mscL Mycobacterium tuberculosis)

Tags

6x His Tag and Thrombin Cleavage site

Expression

Bacterial

Mutation

Changed Glutamic Acid (Glu) 102 to Stop Codon

Promoter

T7 Promoter

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-U6-sgRNA-M5-IRES-CFP

Plasmid

#114728

Purpose

sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M5

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2m

Plasmid

#105066

Purpose

A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2m

Tags

6x His tag

Expression

Bacterial

Promoter

pBAD

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-Tmeff2-2xMyc-EF1-copGFP

Plasmid

#99730

Purpose

overexpression of Tmeff2 and mCherry

Depositor

Mengsheng Qiu

Article

Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

Insert

Tmeff2 (Tmeff2 Mouse)

Tags

2xMyc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;snx5CE1-basEgfp

Plasmid

#90138

Purpose

contains snx5 enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

snx5E1basEgfp (snx5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;cldn5bCE1-basEgfp

Plasmid

#90136

Purpose

contains cldn5b enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

cldn5bE1basEgfp (cldn5b Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;yrkP1Egfp

Plasmid

#90168

Purpose

contains yrk specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

yrkP1Egfp (yrk Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;snx5P1Egfp

Plasmid

#90166

Purpose

contains snx5 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

snx5P1Egfp (snx5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;yrkCE1-P1Egfp

Plasmid

#90156

Purpose

contains yrk enhancer sequence, and yrk specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

yrkE1P1Egfp (yrk Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;snx5CE1-P1Egfp

Plasmid

#90152

Purpose

contains snx5 enhancer sequence, and snx5 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

snx5E1P1Egfp (snx5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;sheCE1-P1Egfp

Plasmid

#90150

Purpose

contains She enhancer sequence, and She specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

sheE1P1Egfp (she Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;yrkCE1-basEgfp

Plasmid

#90142

Purpose

contains yrk enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

yrkE1basEgfp (yrk Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;tmem88aCE1-basEgfp

Plasmid

#90135

Purpose

contains tmem88a enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

tmem88aE1basEgfp (tmem88a Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-WNT1

Plasmid

#79499

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT1 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT1 (WNT1 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pPD3cC

Plasmid

#86972

Purpose

Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

Insert

PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …

Promoter

T7 promoter

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pTIM

Plasmid

#81198

Purpose

pTIM in vitro transcription backbone plasmid with two multiple cloning sites and two Rho-independent transcriptional terminators.

Depositor

Andrés Vázquez-Torres

Article

Tapscott et al Sci Rep. 2018 Jun 21;8(1):9465. doi: 10.

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBB(SnaBI)

Plasmid

#69558

Purpose

In vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Mutation

The two last codons (TATTAA; encoding the last ty…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET23a-MmUBB(Y305P)

Plasmid

#69557

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Mutation

Codon TAT encoding tyrosine 305 was changed to CC…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 FL Q42K C214S

Plasmid

#47089

Depositor

Marina Ramirez-Alvarado

Article

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL Q42K C214S (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Q42K C214S

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only