Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 115,625 results for: mpi

Showing: 5551 - 5600 of 115625 results

  1. pTXB3-Flag PLK1

    Plasmid
    #68271
    Purpose
    bacterial expression of Plk1
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Use
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pcDNA6.MCV.LT206.V5(CM2B4)

    Plasmid
    #28190
    Depositor
    Patrick Moore
    Insert
    MCPyV genomic T antigen locus (206 wild-type strain)
    Use
    Tags
    V5
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    May 24, 2011
    Availability
    Academic Institutions and Nonprofits only
    28190-plasmid-map-sequence-id-424926

    Enlarge

  3. pLNCX chick src Y416F

    Plasmid
    #13662
    Depositor
    Joan Brugge
    Insert
    src Y416F (SRC Chicken)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Y416F
    Promoter
    Available since
    Jan. 4, 2007
    Availability
    Academic Institutions and Nonprofits only

  4. PB CAG-F-bpA-2xSV40pA-F-td-sfGFP

    Plasmid
    #133572
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133572-plasmid-map-sequence-id-274057

    Enlarge

  5. PB CAG-L-bpA-3xSV40pA-L-td-sfGFP

    Plasmid
    #133577
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133577-plasmid-map-sequence-id-274079

    Enlarge

  6. pTRIPZ (M)-YFP-Eed

    Plasmid
    #82512
    Purpose
    Lentivirus vector. Expresses YFP-Eed fusion proteins in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    embryonic ectoderm development
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    insert contains AA39-441 of EeD
    Promoter
    cmv
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82512-plasmid-map-sequence-id-171489

    Enlarge

  7. pUPRT::Floxed DHFR-TS*

    Plasmid
    #100606
    Purpose
    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.
    Depositor
    David Sibley
    Insert
    TgUPRT::Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Pyrimethamine resistance
    Promoter
    pTgDHFR-TS
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    100606-plasmid-map-sequence-id-192862

    Enlarge

  8. PB CAG-F-bpA-3xSV40pA-F-td-sfGFP

    Plasmid
    #133573
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133573-plasmid-map-sequence-id-274063

    Enlarge

  9. pRR2

    Plasmid
    #166097
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166097-plasmid-map-sequence-id-329109

    Enlarge

  10. pET3a-DJ1

    Plasmid
    #51489
    Purpose
    Inducible expression of human DJ1 in E.coli
    Depositor
    Michael J Fox Foundation MJFF
    Insert
    DJ1 (PARK7 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 31, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    51489-plasmid-map-sequence-id-256986

    Enlarge

  11. pAS1-odr-4P::his-24::mNeptune

    Plasmid
    #124342
    Purpose
    Multicolor Worms for Neuronal ID
    Depositor
    Andrew Leifer
    Insert
    odr-4, mNeptune (odr-4 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124342-plasmid-map-sequence-id-241937

    Enlarge

  12. pInt_attP1_ampR

    Plasmid
    #205296
    Purpose
    Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205296-plasmid-map-sequence-id-406997

    Enlarge

  14. PB CAG-L-bpA-2xSV40pA-L-td-sfGFP

    Plasmid
    #133576
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133576-plasmid-map-sequence-id-274071

    Enlarge

  15. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS)

    Plasmid
    #137864
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSAS N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137864-plasmid-map-sequence-id-274459

    Enlarge

  16. PP-NMT

    Plasmid
    #25200
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LmjF32.0080:P5-L421
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pLenti-EF1a-GFP-miRE_shRen-Blast

    Plasmid
    #135682
    Purpose
    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135682-plasmid-map-sequence-id-327602

    Enlarge

  20. pBabepuro-PDGFB

    Plasmid
    #189611
    Purpose
    Retroviral expression vector for PDGFB. stimulates fibroblastic proliferation (Tchenio T, unpublished, 2008)
    Depositor
    Thierry Tchenio
    Insert
    PDGFB (PDGFB Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    SV40 early promoter
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189611-plasmid-map-sequence-id-381075

    Enlarge

  21. pKK1 -pFlp-18::mito4x-GCaMP6f

    Plasmid
    #194969
    Purpose
    Fluorescent reporter of mitochondrial matrix Ca2+.
    Depositor
    Frederic Hoerndli
    Insert
    4 repeats of Cox8 presequence plus GCaMP6f
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Flp-18
    Available since
    June 21, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194969-plasmid-map-sequence-id-388699

    Enlarge

  22. C64m

    Plasmid
    #120980
    Purpose
    MoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).
    Depositor
    Richard Murray
    Insert
    temperature sensitive lambda repressor (cI857)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120980-plasmid-map-sequence-id-236034

    Enlarge

  23. pMZ376

    Plasmid
    #74213
    Purpose
    Combined sgRNA/Cas9 vector with KanMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74213-plasmid-map-sequence-id-139302

    Enlarge

  24. pET-(neg27)GFP-GGS9-Cre-6xHis

    Plasmid
    #89253
    Purpose
    Expresses GFP with -27 theoretical charge fused to Cre in E.Coli
    Depositor
    David Liu
    Insert
    (neg27)GFP-GGS9-Cre-6xHis
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    89253-plasmid-map-sequence-id-176140

    Enlarge

  25. EF1a CHR2 mCherry WPRE

    Plasmid
    #129432
    Purpose
    expression of CHR2 mCherry from EF1a promoter
    Depositor
    Oskar Laur
    Insert
    CHR2 mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129432-plasmid-map-sequence-id-269136

    Enlarge

  27. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid
    #172296
    Purpose
    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing
    Depositor
    Patrik Verstreken
    Insert
    Cas9n-RT-T2A-PuroR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    H840A (Cas9)
    Promoter
    T7 promoter A-initiating
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172296-plasmid-map-sequence-id-340322

    Enlarge

  28. sCFP in pBSDONR P4r-P2

    Plasmid
    #121530
    Purpose
    For making C-terminal sCFP fusions using multisite Gateway cloning
    Depositor
    Roger Innes
    Insert
    sCFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    121530-plasmid-map-sequence-id-234035

    Enlarge

  29. Pk-SOD1

    Plasmid
    #25574
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pk-SOD1
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. pUltra-Smurf-luc-RTTA3

    Plasmid
    #59438
    Purpose
    silence doxycyclin inducible pUltra-dox (also expresses luciferase)
    Depositor
    Kitai Kim, Yildirim Dogan
    Inserts
    luciferase
    Reverse TET-Transactivator 3
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    loxP5171
    Promoter
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    59438-plasmid-map-sequence-id-92494

    Enlarge

  31. pHAGE2-RSV-jGCaMP7s-W

    Plasmid
    #126690
    Purpose
    Mammalian calcium sensor protein.
    Depositor
    Darrell Kotton
    Insert
    jGCaMP7s
    Use
    Lentiviral
    Tags
    6xHIS, T7, Xpress
    Expression
    Mutation
    A52V, A317L
    Promoter
    RSV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126690-plasmid-map-sequence-id-245673

    Enlarge

  32. Col2a1:TFPnls-mErt-Cre-Ert

    Plasmid
    #111152
    Purpose
    Chondrocyte specific inducible Cre
    Depositor
    Elly Tanaka
    Insert
    TFPnls-T2a-mErt-Cre-Ert
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    wt
    Promoter
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    111152-plasmid-map-sequence-id-211539

    Enlarge

  34. pLV Tor1a-V5

    Plasmid
    #175135
    Purpose
    Lentiviral expression of Tor1a-Myc
    Depositor
    Larry Gerace
    Insert
    Tor1a (Tor1a Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175135-plasmid-map-sequence-id-352022

    Enlarge

  35. pHAGE2-RSV-jGCaMP7f-W

    Plasmid
    #126691
    Purpose
    Mammalian calcium sensor protein.
    Depositor
    Darrell Kotton
    Insert
    jGCaMP7f
    Use
    Lentiviral
    Tags
    6xHIS, T7, Xpress
    Expression
    Mutation
    H78K, A317L
    Promoter
    RSV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126691-plasmid-map-sequence-id-245678

    Enlarge

  36. pBluescript mouse ptc

    Plasmid
    #58699
    Purpose
    full length mouse ptc cDNA
    Depositor
    Matthew Scott
    Insert
    ptc (Ptch1 Mouse)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pMC6-TEFkFLP(LYS2)

    Plasmid
    #49458
    Purpose
    overespression of Flp step-arrest mutant in yeast, LYS2 selection
    Depositor
    Marc Gartenberg
    Insert
    Flp(H305L) (flp Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H305L, ‘step-arrest’ mutant,
    Promoter
    TEF1
    Available since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    49458-plasmid-map-sequence-id-73670

    Enlarge

  40. pDEST26-hTLR3-Nluc

    Plasmid
    #197638
    Purpose
    Human CD283 protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TLR3 (TLR3 Human)
    Use
    Tags
    NanoLuciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197638-plasmid-map-sequence-id-392617

    Enlarge

  42. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid
    #172298
    Purpose
    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes
    Depositor
    Patrik Verstreken
    Insert
    CRE-T2A-PuroR
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter A-initiating
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172298-plasmid-map-sequence-id-340323

    Enlarge

  43. L4665

    Plasmid
    #1667
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    myo3-2341, myo3Tsa, IVSA, rf2128, ATG-MitLS, gfpN…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1667-plasmid-map-sequence-id-277

    Enlarge

  44. PB CAG-L-bpA-SV40pA-L-td-sfGFP

    Plasmid
    #133575
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133575-plasmid-map-sequence-id-274068

    Enlarge

  46. PTEN-VC

    Plasmid
    #89332
    Purpose
    expresses hPTEN fused to the C-terminal part of Venus
    Depositor
    Tiago Outeiro
    Insert
    PTEN1 (PTEN Human)
    Use
    Tags
    C-terminal part of Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    89332-plasmid-map-sequence-id-176937

    Enlarge

  47. LHP336 - HA-Ub-K48R

    Plasmid
    #32164
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    K48R
    Promoter
    CUP1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  48. PP-HSP90 (3Q5J)

    Plasmid
    #28281
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-HSP90
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    N-terminal domain
    Promoter
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. pBS mouse GDF7 [TJ#37]

    Plasmid
    #16272
    Depositor
    Thomas Jessell
    Insert
    GDF7 (Gdf7 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 4, 2009
    Availability
    Academic Institutions and Nonprofits only

  50. B0030m_BC

    Plasmid
    #74394
    Purpose
    MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0030 to adjust spacing in MC system. [B:B0030m:C]
    Depositor
    Douglas Densmore
    Insert
    RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    74394-plasmid-map-sequence-id-140417

    Enlarge

Showing: 5551 - 5600 of 115625 results