We narrowed to 2,365 results for: DAP

1. H75K SUMO1

   Plasmid

   #221061

   Purpose

   Expression of H75K SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H75K site mutation on SUMO 1 (SUMO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   H75K

   Promoter

   T7

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. H75S SUMO1

   Plasmid

   #221062

   Purpose

   Expression of H75S SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H75S site mutation on SUMO 1 (SUMO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   H75S

   Promoter

   T7

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA

   Plasmid

   #214614

   Purpose

   adapter for RTK split TEV recruitment assays

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   3xSH2 domains of GRB2-CTEV (GRB2 Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PLC-TIR-GFP

   Plasmid

   #52736

   Purpose

   Retroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker

   Depositor

   Jonathan Kagan

   Article

   Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

   Insert

   Toll/IL-1 Receptor adaptor protein (TIRAP Human)
   

   Use

   Retroviral

   Tags

   GFP and PLCd1 Lipid binding

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-85

   Promoter

   Available Since

   June 18, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pLenti-Cas9-P2A-Puro

   Plasmid

   #110837

   Purpose

   Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

   Plasmid

   #110854

   Purpose

   Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   sgRNA scaffold with spacer
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   U6

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-DAXX-ΔD/E-His

   Plasmid

   #179618

   Purpose

   Express GST-DAXX-ΔDE-His in bacteria

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deleted amino acids 424-495

   Promoter

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-DAXX-D/E-His

   Plasmid

   #179619

   Purpose

   Express DAXX D/E region in bacteria

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Use

   Tags

   GST, His, and TEV

   Expression

   Bacterial

   Mutation

   amino acids 414-505 of DAXX

   Promoter

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOD2045-intControl

   Plasmid

   #100005

   Purpose

   MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Inserts

   zif-1 (zif-1 Nematode)
   unc-119
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFPC1-human APPL1

    Plasmid

    #22198

    Depositor

    Pietro De Camilli

    Article

    Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

    Insert

    Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-ABERA-P2A-Puro

    Plasmid

    #112675

    Purpose

    Lentivirus for constitutive expression of ABE7.10 RA (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    ABERA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. hSUMO1 GFP

    Plasmid

    #118360

    Purpose

    This plasmid expresses the full length human SUMO1.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hSUMO1 (SUMO1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mRFP-human APPL1

    Plasmid

    #22202

    Depositor

    Pietro De Camilli

    Article

    Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

    Insert

    Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBSFI-myr-akt-T308D/S473D

    Plasmid

    #49192

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    T308D; S473D

    Promoter

    T7

    Available Since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti AID-BEmax

    Plasmid

    #157950

    Purpose

    Lentiviral expression of AID-BEmax

    Depositor

    Dali Li

    Article

    Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Lenti AID-BEmax
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-SUMO1

    Plasmid

    #185637

    Purpose

    expression of GST-tagged SUMO1 in bacterial cells

    Depositor

    Irina Bezsonova

    Article

    Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

    Insert

    small ubiquitin-like modifier 1 (SUMO1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac-GST-DAXX-His

    Plasmid

    #175781

    Purpose

    DAXX protein for baculovirus expression system

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpRYc

    Plasmid

    #175575

    Purpose

    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

    Insert

    SpRYc
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    PID of SpRY grafted onto the N-terminal domain of…

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMAL-SH3(2)-5R

    Plasmid

    #112089

    Purpose

    Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-5R (NCK1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    construct contains only repeats of the second SH3…

    Promoter

    Lac

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. hSUMO1-HIS

    Plasmid

    #118361

    Purpose

    This plasmid expresses the full length human SUMO1.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hSUMO1 (SUMO1 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Promoter

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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